调衡方多糖体外增强巨噬细胞吞噬作用
[Macrophage phagocytosis enhanced by Tiaohengfang polysaccharides in vitro].
作者信息
Zhang Hongfang, Yu Penglong, Zheng Yu, Yu Dongbo, Wu Fang, Wang Xisheng, Huan Cheng, Zhang Yimin
机构信息
Shaanxi University of Traditional Chinese Medicine, Xianyang 712046, China. *Corresponding author, E-mail:
Shaanxi University of Traditional Chinese Medicine, Xianyang 712046, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016 Jan;32(1):63-6.
OBJECTIVE
To study the immunoregulatory effect of Tiaohengfang polysaccharides (THFPS) on the phagocytosis of macrophages.
METHODS
According to the preparing method of Chinese medicine polysaccharides, crude polysaccharides were extracted from Tiaohengfang. Mouse peritoneal macrophages were separated and cultured. After the macrophages were treated with (500, 200, 10) μg/mL THFPS for 48 hours, the cell morphology was observed under a light microscope. Phagocytic tests of ink and Staphylococcus aureus were used to evaluate the phagocytosis of the macrophages, and cell enzyme chemical staining was applied to observe the changes of acid phosphatase in macrophages.
RESULTS
Compared with the control group, the volume of macrophages significantly became bigger when they were treated with (500, 200, 10) μg/mL THFPS, the intake of ink and Staphylococcus aureus significantly increased, and the activity of acid phosphatase was also significantly enhanced, which was positively correlated with the dose of THFPS.
CONCLUSION
THFPS can enhance the phagocytosis of macrophages and increase the activity of intracellular acid phosphatase.
目的
研究调衡方多糖（THFPS）对巨噬细胞吞噬功能的免疫调节作用。
方法
按照中药多糖的制备方法，从调衡方中提取粗多糖。分离培养小鼠腹腔巨噬细胞。巨噬细胞用（500、200、10）μg/mL THFPS处理48小时后，在光学显微镜下观察细胞形态。采用墨汁吞噬试验和金黄色葡萄球菌吞噬试验评估巨噬细胞的吞噬功能，并应用细胞酶化学染色观察巨噬细胞酸性磷酸酶的变化。
结果
与对照组相比，（500、200、10）μg/mL THFPS处理后的巨噬细胞体积显著增大，墨汁和金黄色葡萄球菌的摄入量显著增加，酸性磷酸酶活性也显著增强，且与THFPS剂量呈正相关。
结论
THFPS可增强巨噬细胞的吞噬功能，提高细胞内酸性磷酸酶的活性。
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