Revelo Natalia H, Rizzoli Silvio O
Department of Neuro- and Sensory Physiology, University Medical Center Göttingen.
Department of Tumor Immunology, Institute for Molecular Life Sciences, Radboud University Medical Center.
Curr Protoc Neurosci. 2016 Jan 4;74:2.25.1-2.25.21. doi: 10.1002/0471142301.ns0225s74.
mCLING is a fixable endocytosis marker that can be combined with immunolabeling techniques to study the molecular composition of trafficking organelles. mCLING can be used both in cultured cells and in tissue if critical sample preparation steps, such as fixation, are correctly performed. This unit describes protocols for the application of mCLING and for the subsequent sample processing. We include immunostaining protocols and embedding procedures for confocal and high-resolution microscopy.
mCLING是一种可固定的内吞作用标志物，可与免疫标记技术相结合，用于研究运输细胞器的分子组成。如果正确执行关键的样品制备步骤，如固定，mCLING可用于培养细胞和组织。本单元介绍了mCLING的应用及后续样品处理的方案。我们包括用于共聚焦和高分辨率显微镜的免疫染色方案和包埋程序。
