Tielens Sylvia, Godin Juliette D, Nguyen Laurent
GIGA-Neurosciences, University of Liège, Liège, Belgium.
Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, Liège, Belgium.
Curr Protoc Neurosci. 2016 Jan 4;74:3.29.1-3.29.23. doi: 10.1002/0471142301.ns0329s74.
The cerebral cortex is one of the most intricate regions of the brain that requires elaborate cell migration patterns for its development. Experimental observations show that projection neurons migrate radially within the cortical wall, whereas interneurons migrate along multiple tangential paths to reach the developing cortex. Tight regulation of the cell migration processes ensures proper positioning and functional integration of neurons to specific cerebral cortical circuits. Disruption of neuronal migration often leads to cortical dysfunction and/or malformation associated with neurological disorders. Unveiling the molecular control of neuron migration is thus fundamental to understanding the physiological or pathological development of the cerebral cortex. In this unit, protocols allowing detailed analysis of patterns of migration of both interneurons and projection neurons under different experimental conditions (i.e., loss or gain of function) are presented.
大脑皮层是大脑中最复杂的区域之一，其发育需要精细的细胞迁移模式。实验观察表明，投射神经元在皮质壁内沿径向迁移，而中间神经元则沿着多条切线路径迁移以到达发育中的皮层。细胞迁移过程的严格调控确保神经元正确定位并功能整合到特定的大脑皮质回路中。神经元迁移的破坏常常导致与神经疾病相关的皮质功能障碍和/或畸形。因此，揭示神经元迁移的分子控制对于理解大脑皮层的生理或病理发育至关重要。在本单元中，将介绍一些方案，可对不同实验条件下（即功能丧失或功能获得）中间神经元和投射神经元的迁移模式进行详细分析。
