Bolin C A, Zuerner R L, Trueba G
USDA, National Animal Disease Center, Ames, IA 50010.
Am J Vet Res. 1989 Jul;50(7):1001-3.
Nucleic acid hybridization, bacteriologic culture, and a fluorescent antibody test were compared for detection of Leptospira interrogans serovar hardjo type hardjo-bovis in bovine urine. Seventy-five urine samples were collected from pregnant cows challenge exposed with type hardjo-bovis. Twenty samples were collected from steers not exposed to hardjo-bovis. Sediments from each sample were examined, using fluorescent antibodies and a repetitive sequence element nucleic acid probe, to detect the presence of leptospires. Urine samples were processed for bacteriologic culture, using standard techniques. Under laboratory conditions typically used for these techniques, leptospires were detected in 60 of 75 urine samples from challenge exposed cows by nucleic acid hybridization, in 24 samples by fluorescent antibody test, and in 13 samples by bacteriologic culture. Leptospires were not detected in the urine of steers not exposed to hardjo-bovis.
对核酸杂交、细菌培养和荧光抗体检测进行了比较，以检测牛尿液中的问号状钩端螺旋体哈氏血清型哈氏牛型。从用哈氏牛型攻击暴露的怀孕母牛中收集了75份尿液样本。从未暴露于哈氏牛型的公牛中收集了20份样本。使用荧光抗体和重复序列元件核酸探针检查每个样本的沉淀物，以检测钩端螺旋体的存在。使用标准技术对尿液样本进行细菌培养处理。在通常用于这些技术的实验室条件下，通过核酸杂交在75份来自攻击暴露母牛的尿液样本中的60份中检测到钩端螺旋体，通过荧光抗体检测在24份样本中检测到，通过细菌培养在13份样本中检测到。在未暴露于哈氏牛型的公牛尿液中未检测到钩端螺旋体。
