Bolin C A, Thiermann A B, Handsaker A L, Foley J W
USDA, National Animal Disease Center, Ames, IA 50010.
Am J Vet Res. 1989 Jan;50(1):161-5.
Effectiveness of a pentavalent leptospiral vaccine to protect cattle from infection and reproductive problems caused by Leptospira interrogans serovar hardjo type hardjo-bovis was evaluated. Seven cows were vaccinated once and 8 cows were vaccinated twice with a USDA-licensed pentavalent leptospiral vaccine. Five cows were maintained as nonvaccinated controls. Cows were bred 1 to 2 months after the last vaccination. During the 4th to 6th month of gestation, all cows were challenge exposed on 4 occasions by conjunctival instillation of 10(8) serovar hardjo type hardjo-bovis organisms and on 3 occasions by conjunctival instillation of urine from a cow shedding hardjo-bovis. All control cows and 13 of 15 vaccinated cows became infected and shed leptospires in the urine. Leptospires were detected in fewer urine samples collected from vaccinated cows, compared with those collected from control cows. Four stillborn calves and 3 weak calves were born to control and vaccinated cows. Leptospires were detected in the kidneys of 11 apparently healthy calves born to vaccinated and control cows. Agglutinating antibodies were not detected in the precolostral serum of these calves.
评估了一种五价钩端螺旋体疫苗对保护牛免受问号钩端螺旋体哈焦型波摩那亚型感染及生殖问题的有效性。7头母牛接种一次，8头母牛接种两次美国农业部许可的五价钩端螺旋体疫苗。5头母牛作为未接种对照。母牛在最后一次接种后1至2个月进行配种。在妊娠第4至6个月期间，所有母牛通过结膜滴注10(8)个哈焦型波摩那亚型钩端螺旋体菌4次以及通过结膜滴注来自感染波摩那亚型的母牛尿液3次进行攻毒暴露。所有对照母牛和15头接种母牛中的13头被感染并在尿液中排出钩端螺旋体。与对照母牛相比，接种母牛采集的尿液样本中检测到的钩端螺旋体较少。对照母牛和接种母牛产下4头死产犊牛和3头体弱犊牛。在接种母牛和对照母牛产下的11头看似健康的犊牛的肾脏中检测到钩端螺旋体。这些犊牛的初乳前血清中未检测到凝集抗体。
