Wagenaar J A, Segers R P, Van der Zeijst B A
Department of Bacteriology, School of Veterinary Medicine, Utrecht University, The Netherlands.
Mol Biotechnol. 1994 Aug;2(1):1-14. doi: 10.1007/BF02789285.
We have developed an assay for the detection of pathogenic Leptospira that is based on the polymerase chain reaction. With the combination of agarose gel electrophoresis and blotting, pathogenic Leptospira can be discriminated specifically from nonpathogenic Leptospira and other bacterial species. This method, based on the amplification of 16S ribosomal RNA sequences, is able to detect 10 leptospiral cells/mL in cattle urine samples and 100 leptospiral cells/mL in pig urine samples. Using this assay leptospires were detected in urine samples from cattle that were experimentally infected with Leptospira interrogans serovar hardjo type hardjobovis.
我们开发了一种基于聚合酶链反应检测致病性钩端螺旋体的检测方法。通过琼脂糖凝胶电泳和印迹相结合，可将致病性钩端螺旋体与非致病性钩端螺旋体及其他细菌种类特异性区分开来。该方法基于16S核糖体RNA序列的扩增，能够检测牛尿液样本中每毫升10个钩端螺旋体细胞以及猪尿液样本中每毫升100个钩端螺旋体细胞。使用该检测方法，在实验感染问号钩端螺旋体哈焦型哈焦波维斯血清型的牛的尿液样本中检测到了钩端螺旋体。
