Silva Marisa, Rey Verónica, Botana Ana, Vasconcelos Vitor, Botana Luis
Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, Porto 4619-007, Portugal.
Interdisciplinary Center of Marine and Environmental Research-CIMAR/CIIMAR, University of Porto, Rua dos Bragas, 289, Porto 4050-123, Portugal.
Toxins (Basel). 2015 Dec 30;8(1):11. doi: 10.3390/toxins8010011.
Paralytic Shellfish Toxin blooms are common worldwide, which makes their monitoring crucial in the prevention of poisoning incidents. These toxins can be monitored by a variety of techniques, including mouse bioassay, receptor binding assay, and liquid chromatography with either mass spectrometric or pre- or post-column fluorescence detection. The post-column oxidation liquid chromatography with fluorescence detection method, used routinely in our laboratory, has been shown to be a reliable method for monitoring paralytic shellfish toxins in mussel, scallop, oyster and clam species. However, due to its high sensitivity to naturally fluorescent matrix interferences, when working with unconventional matrices, there may be problems in identifying toxins because of naturally fluorescent interferences that co-elute with the toxin peaks. This can lead to erroneous identification. In this study, in order to overcome this challenge in echinoderm and gastropod matrices, we optimized the conversion of Gonyautoxins 1 and 4 to Neosaxitoxin with 2-mercaptoethanol. We present a new and less time-consuming method with a good recovery (82.2%, RSD 1.1%, n = 3), requiring only a single reaction step.
麻痹性贝类毒素藻华在全球范围内很常见，这使得对其进行监测对于预防中毒事件至关重要。这些毒素可以通过多种技术进行监测，包括小鼠生物测定法、受体结合测定法以及液相色谱结合质谱或柱前或柱后荧光检测。我们实验室常规使用的柱后氧化液相色谱荧光检测法已被证明是监测贻贝、扇贝、牡蛎和蛤类中麻痹性贝类毒素的可靠方法。然而，由于其对天然荧光基质干扰的高敏感性，在处理非常规基质时，由于与毒素峰共洗脱的天然荧光干扰，在鉴定毒素时可能会出现问题。这可能导致错误鉴定。在本研究中，为了克服棘皮动物和腹足动物基质中的这一挑战，我们用2-巯基乙醇优化了膝沟藻毒素1和4向新石房蛤毒素的转化。我们提出了一种新的、耗时较少的方法，回收率良好（82.2%，相对标准偏差1.1%，n = 3），只需要一个反应步骤。
