中缝大核中的一氧化氮在低温期间调节大鼠的皮肤血流量。
Nitric oxide in the nucleus raphe magnus modulates cutaneous blood flow in rats during hypothermia.
作者信息
Arami Masoumeh Kourosh, Zade Javad Mirnajafi, Komaki Alireza, Amiri Mahmood, Mehrpooya Sara, Jahanshahi Ali, Jamei Behnam
机构信息
Department of Basic Sciences, Allied Medical College, Iran University of Medical Sciences, Tehran, Iran; Medical College of Saveh, Saveh University of Medical Sciences, Saveh, Iran.
Department of Physiology, Tarbiat Modares University, Tehran, Iran.
出版信息
Iran J Basic Med Sci. 2015 Oct;18(10):989-92.
OBJECTIVES
Nucleus Raphe Magnus (NRM) that is involved in the regulation of body temperature contains nitric oxide (NO) synthase. Considering the effect of NO on skin blood flow control, in this study, we assessed its thermoregulatory role within the raphe magnus.
MATERIALS AND METHODS
To this end, tail blood flow of male Wistar rats was measured by laser doppler following the induction of hypothermia.
RESULTS
Intra-NRM injection of SNP (exogenous NO donor, 0.1- 0.2 μl, 0.2 nM) increased the blood flow. Similarly, unilateral microinjection of glutamate (0.1- 0.2 μl, 2.3 nM) into the nucleus increased the blood flow. This effect of L-glutamate was reduced by prior intra NRM administration of NO synthase inhibitor N(G)-methyl-L-arginine or N(G)-nitro-L-arginine methyl ester (L-NAME, 0.1 µl, 100 nM).
CONCLUSION
It is concluded that NO modulates the thermoregulatory response of NRM to hypothermia and may interact with excitatory amino acids in central skin blood flow regulation.
目的
参与体温调节的中缝大核（NRM）含有一氧化氮（NO）合酶。考虑到NO对皮肤血流控制的影响，在本研究中，我们评估了其在中缝大核内的体温调节作用。
材料与方法
为此，在诱导体温过低后，通过激光多普勒测量雄性Wistar大鼠的尾血流量。
结果
向中缝大核内注射SNP（外源性NO供体，0.1 - 0.2 μl，0.2 nM）可增加血流量。同样，向该核内单侧微量注射谷氨酸（0.1 - 0.2 μl，2.3 nM）也可增加血流量。在中缝大核内预先给予NO合酶抑制剂N(G)-甲基-L-精氨酸或N(G)-硝基-L-精氨酸甲酯（L-NAME，0.1 µl，100 nM）可减弱L-谷氨酸的这种作用。
结论
得出结论，NO调节中缝大核对体温过低的体温调节反应，并且在中枢皮肤血流调节中可能与兴奋性氨基酸相互作用。
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