Chang Hyun-Kyung, Kim Khae Hawn, Kang Ki-Woon, Kang Yoo-Jin, Kim Tae-Wook, Park Hun-Kyung, Kim Sung-Eun, Kim Chang-Ju
Department of Urology, Gachon University Gil Medical Center, Gachon University School of Medicine, Incheon, Korea.
Department of Anesthesiology and Pain Medicine, College of Medicine, Kyung Hee University, Seoul, Korea.
J Exerc Rehabil. 2015 Dec 31;11(6):311-9. doi: 10.12965/jer.150263. eCollection 2015 Dec.
Antidepressants are drugs that relieve symptoms of depressive disorders. Fluoxetine, tianeptine, and milnacipran are different types of antidepressants, and they have widely been used for relieving of depression symptoms. In the present study, the effects of fluoxetine, tianeptine, and milnacipran on the glycine-induced ion current by nystatin-perforated patch clamp and on the amplitude of field potential in the hippocampal CA1 region by multichannel extracellular recording, MED64, system, were studied. In the present results, fluoxetine, tianeptine, and milnacipran reduced glycine-induced ion current in the hippocampal CA1 neurons in nystatin-perforated patch clamp method. These drugs enhanced the amplitude of the field potential in the hippocampal CA1 region in MED64 system. These results suggest that antidepressants may increase neuronal activity by enhancing field potential through inhibition on glycine-induced ion current.
抗抑郁药是缓解抑郁症症状的药物。氟西汀、噻奈普汀和米那普明是不同类型的抗抑郁药，它们已被广泛用于缓解抑郁症状。在本研究中，通过制霉菌素穿孔膜片钳研究了氟西汀、噻奈普汀和米那普明对甘氨酸诱导的离子电流的影响，并通过多通道细胞外记录系统MED64研究了它们对海马CA1区场电位幅度的影响。在本研究结果中，采用制霉菌素穿孔膜片钳法，氟西汀、噻奈普汀和米那普明降低了海马CA1神经元中甘氨酸诱导的离子电流。这些药物增强了MED64系统中海马CA1区的场电位幅度。这些结果表明，抗抑郁药可能通过抑制甘氨酸诱导的离子电流来增强场电位，从而增加神经元活性。
