Śliżewska Katarzyna, Nowak Adriana, Smulikowska Stefania
Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Łódź, Poland.
Polish Academy of Sciences, The Kielanowski Institute of Animal Psysiology and Nutrition, Jabłonna, Poland.
Acta Biochim Pol. 2016;63(2):281-6. doi: 10.18388/abp.2015_1094. Epub 2016 Jan 7.
The aim of the present study was to assess the genotoxicity and cytotoxicity of the faecal water of chickens fed ochratoxin A (OTA) contaminated feed with and without probiotic preparation. The study was performed on 20 healthy female Ross broiler chickens divided into 4 groups: control chickens - fed with non-supplemented feed; PP chickens - fed feed supplemented with the probiotic preparation; OTA chickens - fed feed contaminated with 1 mg per kg of OTA; OTA + PP chickens - fed feed contaminated with 1 mg per kg of OTA and supplemented with the probiotic preparation. Faecal water samples were collected on the 35(th) day of life of chickens from each group. Genotoxicity was measured using the comet assay, and cytotoxicity by means of MTT tests. Mean DNA damage, measured as the percentage of DNA in the tails of the comets, was 8.50 ± 1.10 for chickens fed OTA at 1 mg/kg and 6.41 ± 0.67 in the controls. The supplementation of feed with the probiotic preparation decreased the extent of DNA damage to 4.74 ± 0.78. In the control group of chickens the average cytotoxicity was 38.5 ± 0.5 (in MTT), while in the probiotic preparation group (PP group) it was 31.8 ± 0.7 (in MTT). After supplementation of the feed with the probiotic preparation, the genotoxicity and cytotoxicity were decreased in a statistically significant manner.
本研究的目的是评估在饲喂含或不含益生菌制剂的赭曲霉毒素A（OTA）污染饲料的情况下，鸡粪便水的遗传毒性和细胞毒性。该研究对20只健康的雌性罗斯肉鸡进行，分为4组：对照鸡——饲喂未添加任何物质的饲料；PP鸡——饲喂添加了益生菌制剂的饲料；OTA鸡——饲喂每千克含1毫克OTA的污染饲料；OTA + PP鸡——饲喂每千克含1毫克OTA且添加了益生菌制剂的污染饲料。在鸡出生第35天收集每组鸡的粪便水样。使用彗星试验测量遗传毒性，通过MTT试验测量细胞毒性。以彗星尾部DNA百分比衡量的平均DNA损伤，对于饲喂每千克1毫克OTA的鸡为8.50±1.10，对照组为6.41±0.67。在饲料中添加益生菌制剂可将DNA损伤程度降低至4.74±0.78。在对照组鸡中，平均细胞毒性为38.5±0.5（MTT法），而在益生菌制剂组（PP组）中为31.8±0.7（MTT法）。在饲料中添加益生菌制剂后，遗传毒性和细胞毒性均有统计学意义的降低。
