Truman P, Pomare L, Ford H C
Department of Pathology, Wellington School of Medicine, Wellington Hospital, New Zealand.
Arch Gynecol Obstet. 1989;246(1):39-49. doi: 10.1007/BF00933076.
Methods of disaggregation of human placental tissue were assessed with the aim of maximising the yield of cytotrophoblast cells and minimising contamination with other cell types. Brief exposure to crude trypsin was found to be the best way to balance yield of trophoblast cells against contamination by cells of the villous core. Much higher yields of all cell types could be obtained by digestion with other enzymes. Staining for NADH diaphorase activity coupled with general morphology was found to be a reasonably specific, rapid and simple method of distinguishing cytotrophoblast cells in disaggregated mixtures. Alkaline phosphatase activity was an unreliable marker of trophoblast tissue in early placentas, and of the putative cytotrophoblast cells in mixtures of disaggregated cells. Cultures of cells obtained from term placentas were fairly homogeneous, whereas placentas of 6-12 weeks gestation gave heterogeneous cell cultures which became overgrown with fibroblasts.
为了使细胞滋养层细胞的产量最大化并尽量减少其他细胞类型的污染，对人胎盘组织的解离方法进行了评估。发现短暂暴露于粗胰蛋白酶是平衡滋养层细胞产量与绒毛核心细胞污染的最佳方法。用其他酶消化可获得所有细胞类型高得多的产量。发现结合一般形态学对NADH黄递酶活性进行染色是在解离混合物中区分细胞滋养层细胞的一种相当特异、快速且简单的方法。碱性磷酸酶活性在早期胎盘的滋养层组织以及解离细胞混合物中假定的细胞滋养层细胞中是不可靠的标志物。足月胎盘获得的细胞培养物相当均匀，而妊娠6至12周的胎盘产生的细胞培养物异质性较高，且会被成纤维细胞过度生长。
