Cai X Y, Redfield B, Maxon M, Weissbach H, Brot N
Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.
Biochem Biophys Res Commun. 1989 Aug 30;163(1):79-83. doi: 10.1016/0006-291x(89)92101-3.
An Escherichia coli S-30 DNA directed protein synthesis system was used to study the effect of homocysteine on the in vitro expression of the metE, metH and metR genes. In the presence of purified MetR protein, which is known to regulate the expression of these genes, homocysteine activates metE expression and inhibits both metR and metH expression. These findings support the recent in vivo results of Urbanowski, M.L. and Stauffer, G.V. (1989), J. Bacteriol. 171, 3277-3281.
利用大肠杆菌S-30 DNA指导的蛋白质合成系统，研究同型半胱氨酸对metE、metH和metR基因体外表达的影响。已知纯化的MetR蛋白可调节这些基因的表达，在其存在的情况下，同型半胱氨酸激活metE表达，并抑制metR和metH表达。这些发现支持了Urbanowski, M.L.和Stauffer, G.V.（1989年）最近的体内研究结果，《细菌学杂志》171, 3277 - 3281。
