Cai X Y, Maxon M E, Redfield B, Glass R, Brot N, Weissbach H
Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.
Proc Natl Acad Sci U S A. 1989 Jun;86(12):4407-11. doi: 10.1073/pnas.86.12.4407.
Studies by Urbanowski et al. [Urbanowski, M. L., Stauffer, L. T., Plamann, L. S. & Stauffer, G. V. (1987) J. Bacteriol. 169, 1391-1397] have identified a regulatory locus, called metR, required for the expression of the metE and metH genes. We recently purified the MetR protein from Escherichia coli and showed that it could stimulate the in vitro expression of the metE gene and autoregulate its own synthesis. In the present study, the purified MetR protein has been shown to stimulate the in vitro expression of the metH gene. Also, the in vitro synthesized MetE, MetH, and MetR proteins were shown to be biologically active. The transcription start sites for the metE and metR genes have been determined, and DNA footprinting experiments have identified regions in the metE-metR intergenic sequence that are protected by either the MetR or MetJ proteins.
乌尔巴诺夫斯基等人[乌尔巴诺夫斯基，M. L.，斯托弗，L. T.，普拉曼，L. S. & 斯托弗，G. V.（1987年）《细菌学杂志》169卷，1391 - 1397页]的研究确定了一个名为metR的调控位点，它是metE和metH基因表达所必需的。我们最近从大肠杆菌中纯化了MetR蛋白，并表明它可以刺激metE基因的体外表达并自动调节其自身的合成。在本研究中，纯化的MetR蛋白已被证明可以刺激metH基因的体外表达。此外，体外合成的MetE、MetH和MetR蛋白被证明具有生物活性。已确定了metE和metR基因的转录起始位点，并且DNA足迹实验已确定了metE - metR基因间序列中受MetR或MetJ蛋白保护的区域。
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