Yang Gordon C C, Huang Sheng-Chih, Jen Yu-Sheng, Tsai Pei-Shin
Center for Emerging Contaminants Research, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan, ROC; Institute of Environmental Engineering, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan, ROC.
Institute of Environmental Engineering, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan, ROC.
Chemosphere. 2016 May;150:576-585. doi: 10.1016/j.chemosphere.2015.12.044. Epub 2015 Dec 28.
The objective of this study was to evaluate the feasibility of enhanced bioremediation coupling with electrokinetic process for promoting the growth of intrinsic microorganisms and removing phthalate esters (PAEs) from river sediment by adding an oxygen releasing compound (ORC). Test results are given as follows: Enhanced removal of PAEs was obtained by electrokinetics, through which the electroosmotic flow would render desorption of organic pollutants from sediment particles yielding an increased bioavailability. It was also found that the ORC injected into the sediment compartment not only would alleviate the pH value variation due to acid front and base front, but would be directly utilized as the carbon source and oxygen source for microbial growth resulting in an enhanced degradation of organic pollutants. However, injection of the ORC into the anode compartment could yield a lower degree of microbial growth due to the loss of ORC during the transport by EK. Through the analysis of molecular biotechnology it was found that both addition of an ORC and application of an external electric field can be beneficial to the growth of intrinsic microbial and abundance of microflora. In addition, the sequencing result showed that PAEs could be degraded by the following four strains: Flavobacterium sp., Bacillus sp., Pseudomonas sp., and Rhodococcus sp. The above findings confirm that coupling of enhanced bioremediation and electrokinetic process could be a viable remediation technology to treat PAEs-contaminated river sediment.
本研究的目的是评估强化生物修复与电动过程相结合的可行性，通过添加释氧化合物（ORC）来促进内源微生物的生长并从河流沉积物中去除邻苯二甲酸酯（PAEs）。测试结果如下：电动过程实现了PAEs的强化去除，电渗流使有机污染物从沉积物颗粒上解吸，从而提高了生物可利用性。还发现注入沉积物隔室的ORC不仅能缓解由于酸前沿和碱前沿引起的pH值变化，还能直接作为微生物生长的碳源和氧源，从而增强有机污染物的降解。然而，由于ORC在电动传输过程中的损失，将其注入阳极隔室会导致微生物生长程度较低。通过分子生物技术分析发现，添加ORC和施加外部电场都有利于内源微生物的生长和微生物群落的丰度。此外，测序结果表明，PAEs可被以下四种菌株降解：黄杆菌属、芽孢杆菌属、假单胞菌属和红球菌属。上述研究结果证实，强化生物修复与电动过程相结合可能是一种可行的修复技术，用于处理受PAEs污染的河流沉积物。
