侵染中国甜樱桃的小樱桃病毒1（LChV-1）的全核苷酸序列
Complete nucleotide sequence of little cherry virus 1 (LChV-1) infecting sweet cherry in China.
作者信息
Wang Jiawei, Zhu Dongzi, Tan Yue, Zong Xiaojuan, Wei Hairong, Hammond Rosemarie W, Liu Qingzhong
机构信息
Key Laboratory for Fruit Biotechnology Breeding of Shandong Province, Shandong Institute of Pomology, Taian, 271000, Shandong, People's Republic of China.
US Department of Agriculture, ARS, Molecular Plant Pathology Laboratory, Room 214, Building 004 BARC West, Beltsville, MD, 20705, USA.
出版信息
Arch Virol. 2016 Mar;161(3):749-53. doi: 10.1007/s00705-015-2737-z. Epub 2016 Jan 5.
Little cherry virus 1 (LChV-1), associated with little cherry disease (LCD), has a significant impact on fruit quality of infected sweet cherry trees. We report the full genome sequence of an isolate of LChV-1 from Taian, China (LChV-1-TA), detected by small-RNA deep sequencing and amplified by overlapping RT-PCR. The LChV-1-TA genome was 16,932 nt in length and contained nine open reading frames (ORFs), with sequence identity at the overall genome level of 76%, 76%, and 78% to LChV-1 isolates Y10237 (UW2 isolate), EU715989 (ITMAR isolate) and JX669615 (V2356 isolate), respectively. Based on the phylogenetic analysis of HSP70h amino acid sequences of Closteroviridae family members, LChV-1-TA was grouped into a well-supported cluster with the members of the genus Velarivirus and was also closely related to other LChV-1 isolates. This is the first report of the complete nucleotide sequence of LChV-1 infecting sweet cherry in China.
与小樱桃病（LCD）相关的小樱桃病毒1（LChV-1）对感染的甜樱桃树的果实品质有重大影响。我们报告了一株来自中国泰安的LChV-1分离株（LChV-1-TA）的全基因组序列，该序列通过小RNA深度测序检测，并通过重叠RT-PCR扩增。LChV-1-TA基因组长度为16,932 nt，包含9个开放阅读框（ORF），在全基因组水平上与LChV-1分离株Y10237（UW2分离株）、EU715989（ITMAR分离株）和JX669615（V2356分离株）的序列同一性分别为76%、76%和78%。基于对Closteroviridae家族成员HSP70h氨基酸序列的系统发育分析，LChV-1-TA与Velarivirus属的成员归为一个得到充分支持的聚类，并且也与其他LChV-1分离株密切相关。这是中国首次报道感染甜樱桃的LChV-1的完整核苷酸序列。
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