Bercier Marjorie, Langlois Isabelle, Dunn Marilyn, Hélie Pierre, Burns Patrick, Gara-Boivin Carolyn
Department of Clinical Sciences (Bercier, Langlois, Dunn, Burns) and Department of Pathology and Microbiology (Gara-Boivin, Hélie), Faculty of Veterinary Medicine, University of Montreal, St. Hyacinthe, Quebec J2S 2M2.
Can J Vet Res. 2016 Jan;80(1):74-80.
The objective of this study was to investigate the normal cytological evaluation of bronchoalveolar lavage (BAL) fluid in healthy adult ferrets (N = 12). These ferrets underwent bronchoscopy and BAL using sterile saline [1.5 mL/kg body weight (BW)]. Percentage of fluid recovered, total leukocyte count, differential leukocyte count, and cell count of the epithelial lining fluid (ELF) were determined. The mean percentage of lavage volume recovered from the right lung and left lung were 67.8 ± 14.9% and 69.7 ± 20.0%, respectively. Gender (P = 0.12) and weight (P = 0.17) did not significantly affect the mean percentage of recovered volume. The mean percentage of recovered volume (P = 0.47) and the mean leukocyte count (P = 0.17) from the right and left lung were not significantly different. Macrophages were the main leukocyte component of the lavages, followed by neutrophils, lymphocytes, and eosinophils. The mean proportion of ELF in BAL fluid was 9.3 ± 3.7% v/v. Bronchoscopy is clinically useful for collecting good quality BAL samples for cytological analysis in ferrets. The leucocyte differential was established, which may help veterinarians to make better clinical decisions when treating respiratory disease. Further studies are required with a larger group in order to establish the healthy reference intervals for BAL values in ferrets.
本研究的目的是调查健康成年雪貂（N = 12）支气管肺泡灌洗（BAL）液的正常细胞学评估。这些雪貂接受了支气管镜检查并用无菌盐水[1.5 mL/kg体重（BW）]进行BAL。测定回收液体的百分比、总白细胞计数、白细胞分类计数以及上皮衬液（ELF）的细胞计数。从右肺和左肺回收的灌洗体积的平均百分比分别为67.8±14.9%和69.7±20.0%。性别（P = 0.12）和体重（P = 0.17）对回收体积的平均百分比没有显著影响。右肺和左肺的回收体积平均百分比（P = 0.47）和平均白细胞计数（P = 0.17）没有显著差异。巨噬细胞是灌洗液中的主要白细胞成分，其次是中性粒细胞、淋巴细胞和嗜酸性粒细胞。BAL液中ELF的平均比例为9.3±3.7%（v/v）。支气管镜检查在临床上有助于为雪貂的细胞学分析采集高质量的BAL样本。建立了白细胞分类，这可能有助于兽医在治疗呼吸道疾病时做出更好的临床决策。需要对更大的群体进行进一步研究，以建立雪貂BAL值的健康参考区间。
