Mills Paul C, Litster Annette
School of Veterinary Science, University of Queensland, Brisbane, Australia.
J Feline Med Surg. 2006 Apr;8(2):105-10. doi: 10.1016/j.jfms.2005.11.001. Epub 2005 Dec 27.
A technique to standardise the analysis of cellular and non-cellular components in epithelial lining fluid (ELF) collected during saline lavage of pulmonary and pleural cavities was developed using the urea dilution method. Bronchoalveolar lavage (BAL) and pleural lavage (PL) fluids were collected from 12 clinically healthy cats. Total and differential cell counts in BAL fluid were within normal ranges for the cat, while cell counts in PL fluid were assumed to be normal based on clinical health during examination, auscultation and lactate dehydrogenase (LDH) activities being comparable with other species. The major clinical implication of this study was that nucleated cell counts within feline ELF could not be predicted from analysis of lavage fluid which suggests that calculation of the proportion of ELF in lavage fluid by the urea dilution method may be necessary to avoid misdiagnosis of health or disease in pulmonary or pleural cavities.
采用尿素稀释法开发了一种标准化分析在肺和胸膜腔盐水灌洗期间收集的上皮衬液（ELF）中细胞和非细胞成分的技术。从12只临床健康的猫收集支气管肺泡灌洗（BAL）液和胸膜灌洗（PL）液。BAL液中的总细胞计数和分类细胞计数在猫的正常范围内，而PL液中的细胞计数根据检查、听诊期间的临床健康状况以及乳酸脱氢酶（LDH）活性与其他物种相当而被假定为正常。本研究的主要临床意义在于，不能通过灌洗液分析来预测猫ELF中的有核细胞计数，这表明可能有必要通过尿素稀释法计算灌洗液中ELF的比例，以避免对肺或胸膜腔的健康或疾病进行误诊。
