兔小肠蔗糖酶-异麦芽糖酶复合物的纯化：与其他麦芽糖酶的分离。
Purification of the rabbit small intestinal sucrase-isomaltase complex: separation from other maltases.
作者信息
Vasseur M
机构信息
Centre de Recherches sur la Nutrition, Centre National de la Recherche Scientifique, Meudon, France.
出版信息
Biosci Rep. 1989 Jun;9(3):341-6. doi: 10.1007/BF01114686.
The rabbit intestinal sucrase-isomaltase complex has been purified to homogeneity after solubilization with Triton X 100 followed by chromatography on DEAE Sepharose CL 6B and a second solubilization with papain. After hydrophobic chromatography on Octyl Sepharose CL 6B, separation from other contaminating maltases was achieved by gel filtration on Ultrogel ACA 22. The final enzyme was purified 390 fold, with a specific activity of about 10 units per mg protein.
兔肠蔗糖酶-异麦芽糖酶复合物先用Triton X 100增溶，然后在DEAE琼脂糖CL 6B上进行层析，再用木瓜蛋白酶进行第二次增溶，之后已被纯化至同质。在辛基琼脂糖CL 6B上进行疏水层析后，通过在Ultrogel ACA 22上进行凝胶过滤，与其他污染性麦芽糖酶分离。最终的酶被纯化了390倍，比活性约为每毫克蛋白质10个单位。
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