Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Biosens Bioelectron. 2016 May 15;79:379-85. doi: 10.1016/j.bios.2015.12.080. Epub 2015 Dec 23.
In this work, silver nanoparticles-doped Pb (II) metal-organic framework (Ag-MOF) was prepared and exploited as a luminescence probe for the development of label-free electrochemiluminescence (ECL) immunosensing scheme for prostate-specific antigen (PSA). The β-cyclodextrin based MOF, Pb-β-cyclodextrin (Pb(II)-β-CD) shows excellent ECL behavior and unexpected reducing capacity towards silver ions. Silver nanoparticles could massively form on the surface of Pb(II)-β-CD (Ag@Pb(II)-β-CD) without use any additional reducing agent, while the ECL behavior of Pb(II)-β-CD still was well retained. The Ag@Pb(II)-β-CD was used as a substrate material to modify glass carbon electrodes and formed a sensing platform for the fabricating ECL immunosensor. The presence of silver nanoparticles enables the facile immobilization of capturing antibody of PSA. The specific binding of PSA onto the electrode surface induces the decrease of ECL signals. A linear range of 0.001-50 ng mL(-1) with a detection limit of 0.34 pg mL(-1) (S/N=3) was obtained after the optimization of experimental conditions. This simply fabricated immunosensor exhibits good stability, accuracy and acceptable reproducibility, which suggesting its potential applications in clinical diagnostics.
在这项工作中，制备了银纳米粒子掺杂的 Pb(II) 金属有机骨架（Ag-MOF），并将其开发为用于前列腺特异性抗原（PSA）的无标记电化学发光（ECL）免疫传感方案的荧光探针。基于 β-环糊精的 MOF，Pb-β-环糊精（Pb(II)-β-CD）表现出优异的 ECL 行为和对银离子的意外还原能力。银纳米粒子可以在 Pb(II)-β-CD（Ag@Pb(II)-β-CD）的表面大量形成，而无需使用任何额外的还原剂，同时 Pb(II)-β-CD 的 ECL 行为仍得到很好的保留。Ag@Pb(II)-β-CD 被用作修饰玻碳电极的基底材料，并形成用于制备 ECL 免疫传感器的传感平台。银纳米粒子的存在使得 PSA 的捕获抗体能够轻易地固定化。PSA 特异性结合到电极表面会导致 ECL 信号降低。经过实验条件的优化，获得了 0.001-50ng mL(-1) 的线性范围和 0.34pg mL(-1)（S/N=3）的检测限。这种简单制备的免疫传感器表现出良好的稳定性、准确性和可接受的重现性，表明其在临床诊断中的潜在应用。
