State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China.
State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China.
Colloids Surf B Biointerfaces. 2022 Oct;218:112718. doi: 10.1016/j.colsurfb.2022.112718. Epub 2022 Jul 23.
A sandwich electrochemiluminescence (ECL) immunosensor based on an N-(4-aminobutyl)-N-ethylisoluminol-graphene oxide-Ag nanoparticle (ABEI-GO-AgNPs) complex and cysteine silver nanowires (AgCysNWs) was prepared to detect prostate-specific antigen (PSA). Our results showed that an ECL signal probe, ABEI-GO-AgNPs, with an ultrahigh specific surface area, favorable catalytic properties, and electrical conductivity was prepared by a one-step synthesis method. ABEI-GO-AgNPs with good biocompatibility immobilized secondary antibody (Ab) via AgN bonds. Furthermore, AgCysNWs containing many -COOH groups were prepared and used to enrich primary antibody (Ab), which could be used as an affinity probe for the selective capture of PSA. Lastly, through layer-by-layer assembly, we established an ECL immunosensing platform for the sensitive detection of PSA. Under the optimized conditions, the designed ECL immunosensor showed promising sensitivity and selectivity for the detection of PSA in the linear range of 5.5 × 10-5.5 ng/mL, with a detection limit of 1.2 × 10 ng/mL. The constructed ECL sensing platform possessed good specificity, reproducibility, and stability and could detect PSA in actual human serum samples.
基于 N-(4-氨基丁基)-N-乙基异鲁米诺-氧化石墨烯-银纳米粒子（ABEI-GO-AgNPs）复合物和半胱氨酸银纳米线（AgCysNWs）的三明治型电化学发光（ECL）免疫传感器被制备用于检测前列腺特异性抗原（PSA）。我们的结果表明，通过一步合成法制备了具有超高比表面积、良好催化性能和导电性的 ECL 信号探针 ABEI-GO-AgNPs。具有良好生物相容性的 ABEI-GO-AgNPs 通过 AgN 键固定了二级抗体（Ab）。此外，还制备了含有许多-COOH 基团的 AgCysNWs，用于富集一级抗体（Ab），它可以作为 PSA 选择性捕获的亲和探针。最后，通过层层组装，我们建立了用于 PSA 灵敏检测的 ECL 免疫传感平台。在优化条件下，所设计的 ECL 免疫传感器在 5.5×10-5.5ng/mL 的线性范围内对 PSA 表现出良好的灵敏度和选择性，检测限为 1.2×10ng/mL。所构建的 ECL 传感平台具有良好的特异性、重现性和稳定性，可用于检测实际人血清样品中的 PSA。
