Department of Chemical Engineering, Faculty of Engineering, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Department of Computer Engineering, Faculty of Engineering, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Bioresour Technol. 2016 Mar;203:252-8. doi: 10.1016/j.biortech.2015.12.065. Epub 2015 Dec 24.
Pilot-scale steam explosion equipments were designed and constructed, to experimentally solubilize xylose from oil palm empty fruit bunches (OPEFB) and also to enhance an enzyme accessibility of the residual cellulose pulp. The OPEFB was chemically pretreated prior to steam explosion at saturated steam (SS) and superheated steam (SHS) conditions. The acid pretreated OPEFB gave the highest xylose recovery of 87.58 ± 0.21 g/kg dried OPEFB in the liquid fraction after explosion at SHS condition. These conditions also gave the residual cellulose pulp with high enzymatic accessibility of 73.54 ± 0.41%, which is approximately threefold that of untreated OPEFB. This study has shown that the acid pretreatment prior to SHS explosion is an effective method to enhance both xylose extraction and enzyme accessibility of the exploded OPEFB. Moreover, the xylose solution obtained in this manner could directly be fermented by Candida shehatae TISTR 5843 giving high ethanol yield of 0.30 ± 0.08 g/g xylose.
设计和构建了中试规模的蒸汽爆炸设备，以实验性地从油棕空果串（OPEFB）中溶解木糖，并提高残余纤维素纸浆的酶可及性。在饱和蒸汽（SS）和过热蒸汽（SHS）条件下进行蒸汽爆炸之前，对 OPEFB 进行了化学预处理。酸预处理的 OPEFB 在 SHS 条件下爆炸后，液体部分的木糖收率最高，为 87.58 ± 0.21 g/kg 干燥的 OPEFB。这些条件还使残余纤维素纸浆具有高的酶可及性，为 73.54 ± 0.41%，约为未处理的 OPEFB 的三倍。这项研究表明，在 SHS 爆炸之前进行酸预处理是一种有效方法，可以提高木糖的提取效率和爆炸后的 OPEFB 的酶可及性。此外，通过这种方式获得的木糖溶液可以直接被 Candida shehatae TISTR 5843 发酵，木糖的乙醇产率为 0.30 ± 0.08 g/g。
