Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia; Research Centre for Sustainable Process Technology, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia.
Bioresour Technol. 2016 Jul;211:200-8. doi: 10.1016/j.biortech.2016.02.135. Epub 2016 Mar 4.
Oil palm empty fruit bunch (OPEFB) fibre is widely available in Southeast Asian countries and found to have 60% (w/w) sugar components. OPEFB was pretreated using the ammonia fibre expansion (AFEX) method and characterised physically by the Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy. The results show that there were significant structural changes in OPEFB after the pretreatment step, and the sugar yield after enzymatic hydrolysis using a cocktail of Cellic Ctec2® and Cellic Htec2® increased from 0.15gg(-1) of OPEFB in the raw untreated OPEFB sample to 0.53gg(-1) of OPEFB in AFEX-pretreated OPEFB (i.e. almost a fourfold increase in sugar conversion), which enhances the economic value of OPEFB. A biohydrogen fermentability test of this hydrolysate was carried out using a locally isolated bacterium, Enterobacter sp. KBH6958. The biohydrogen yield after 72h of fermentation was 1.68mol H2 per mol sugar. Butyrate, ethanol, and acetate were the major metabolites.
油棕空果串（OPEFB）纤维在东南亚国家广泛存在，并且发现其含有 60%（w/w）的糖份。OPEFB 采用氨纤维膨胀（AFEX）方法进行预处理，并通过傅里叶变换红外光谱、X 射线衍射和扫描电子显微镜对其物理性质进行了表征。结果表明，预处理后 OPEFB 的结构发生了显著变化，使用 Cellic Ctec2®和 Cellic Htec2®混合酶进行酶水解后的糖产量从原始未处理的 OPEFB 样品中的 0.15gg（-1）增加到 AFEX 预处理的 OPEFB 中的 0.53gg（-1）（即糖转化率几乎增加了四倍），从而提高了 OPEFB 的经济价值。使用本地分离的细菌 Enterobacter sp. KBH6958 对该水解物进行了生物制氢发酵试验。发酵 72 小时后的生物制氢产量为每摩尔糖 1.68 摩尔 H2。丁酸、乙醇和乙酸是主要的代谢产物。
