使用微流控装置构建稳定的毛细血管网络。
Construction of stable capillary networks using a microfluidic device.
作者信息
Sudo Ryo
出版信息
Annu Int Conf IEEE Eng Med Biol Soc. 2015;2015:350-3. doi: 10.1109/EMBC.2015.7318371.
Construction of stable capillary networks is required to provide sufficient oxygen and nutrients to the deep region of thick tissues, which is important in the context of 3D tissue engineering. Although conventional in vitro culture models have been used to investigate the mechanism of capillary formation, recent advances in microfluidics technologies allowed us to control biophysical and biochemical culture environments more precisely, which led to the construction of functional and stable capillary networks. In this study, endothelial cells and mesenchymal stem cells were co-cultured in microfluidic devices to construct stable capillary networks, which resulted in the construction of luminal structures covered by pericytes. Interactions between endothelial cells and mesenchymal stem cells are also discussed in the context of capillary formation.
构建稳定的毛细血管网络对于向厚组织的深部区域提供充足的氧气和营养物质至关重要，这在三维组织工程中具有重要意义。尽管传统的体外培养模型已被用于研究毛细血管形成的机制，但微流控技术的最新进展使我们能够更精确地控制生物物理和生化培养环境，从而构建出功能性和稳定的毛细血管网络。在本研究中，内皮细胞和间充质干细胞在微流控装置中共同培养以构建稳定的毛细血管网络，结果形成了被周细胞覆盖的管腔结构。还在毛细血管形成的背景下讨论了内皮细胞与间充质干细胞之间的相互作用。
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