Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry , Tsinghua University , Beijing , 100084 , China.
CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience , National Center for Nanoscience and Technology , Beijing 100190 , China.
Anal Chem. 2019 Oct 1;91(19):12283-12289. doi: 10.1021/acs.analchem.9b02434. Epub 2019 Sep 10.
Three-dimensional (3D) hydrogel microspheres have aroused increasing attention as an in vitro cell culture model. Yet the preservation of cells' original biological properties has been overlooked during model construction. Here we present an integrated microfluidic device to accomplish the overall process including cell-laden microsphere generation, online extraction, and dynamic-culture. The method extends the noninvasive and nonsuppression capabilities of the droplet preparation system and provides a constant microenvironment, which reduces intracellular oxidative stress damage and the accumulation of mitochondria. Compared to the conventional preparation method, the coculture model of tumor-endothelial construction on an integrated platform displays high-level angiogenic protein expression. We believe that this versatile and biocompatible platform will provide a more reliable analysis tool for tissue engineering and cancer therapy.
三维（3D）水凝胶微球作为一种体外细胞培养模型受到了越来越多的关注。然而，在模型构建过程中，细胞原始生物特性的保存被忽视了。在这里，我们提出了一种集成微流控装置来完成包括细胞加载微球生成、在线提取和动态培养在内的整个过程。该方法扩展了液滴制备系统的非侵入性和非抑制性能力，并提供了一个恒定的微环境，减少了细胞内氧化应激损伤和线粒体的积累。与传统的制备方法相比，在集成平台上构建的肿瘤内皮共培养模型显示出高水平的血管生成蛋白表达。我们相信，这种多功能和生物相容性的平台将为组织工程和癌症治疗提供更可靠的分析工具。
