Gnutti Alessandro, Signoroni Alberto, Leonardi Riccardo, Corsini Michela, Presta Marco, Mitola Stefania
Annu Int Conf IEEE Eng Med Biol Soc. 2015 Aug;2015:763-6. doi: 10.1109/EMBC.2015.7318474.
Angiogenesis, the process of new blood vessels formation, plays a key role in different physiological and pathological conditions and it is considered a promising target for the development of new anti-inflammatory and anti-tumor therapies. Several assays have been developed to mimic the angiogenic process in vitro and in vivo. Here we propose a technique for the quantification of the pro-angiogenic or anti-angiogenic responses induced by different molecules when implanted in vivo on the chick embryo chorioallantoic membrane (CAM). At day 11 of development CAM is completely vascularized and neo-vessels induced by exogenous molecules converge radially to the implant. Our algorithm is an effective and rapid tool to characterize molecules endowed with proor anti-angiogenic effects by means of the quantification of the vessels present in the CAM macroscopic images. Based on conventional and dedicated image morphology tools, the proposed technique is able to discriminate radial from non-radial vessels, excluding the last ones from the count.
血管生成，即新血管形成的过程，在不同的生理和病理状况中发挥着关键作用，并且它被认为是开发新型抗炎和抗肿瘤疗法的一个有前景的靶点。已经开发了几种体外和体内模拟血管生成过程的检测方法。在此，我们提出一种技术，用于量化不同分子植入鸡胚绒毛尿囊膜（CAM）体内时所诱导的促血管生成或抗血管生成反应。在发育的第11天，CAM完全血管化，外源性分子诱导的新血管呈放射状汇聚到植入物处。我们的算法是一种有效且快速的工具，通过量化CAM宏观图像中存在的血管来表征具有促血管生成或抗血管生成作用的分子。基于传统和专用的图像形态学工具，所提出的技术能够区分放射状血管和非放射状血管，在计数时排除后者。
