Puscz Flemming, Hatem Noah Jozsef, Schmidt Sonja Verena, Reinkemeier Felix, Drysch Marius, Becerikli Mustafa, Steubing Yonca, Lehnhardt Marcus, Wallner Christoph
Department of Plastic Surgery, BG University Hospital Bergmannsheil, Ruhr University Bochum, 44789 Bochum, Germany.
Int J Mol Sci. 2025 May 31;26(11):5318. doi: 10.3390/ijms26115318.
Fibrosarcomas represent a rare but highly aggressive tumor entity among soft tissue tumors. Due to its rarity, questions regarding its development and pathophysiology remain unclear. The chorioallantoic membrane (CAM) assay represents an easily available method to investigate tumors on a growth membrane, live and in ovo. The following study was established to test whether the growth of fibrosarcoma spheroids on the CAM was possible and to critically review their applicability for downstream investigations. The shells of fertilized chicken eggs were opened and the previously prepared HT1080 cell spheroids (50,000, 75,000, and 100,000 cells per spheroid) were applied to the CAM. After 7 days, tumors were examined for size, weight, and vascularization. After 7 days, 80 of 163 chicken eggs showed sufficient tumor growth. Of these 80 eggs with confirmed tumor growth, 32 (40%) were from the 50,000 spheroid group, 18 (22.5%) were from the 75,000 spheroid group, and 30 (37.5%) were from the 100,000 spheroid group. The 100,000-cell spheroid group exhibited the highest weights, with a mean of 110.7 mg, as well as tumor size expansion. This cell number also showed the highest vascularization rates. Tumor growth of fibrosarcoma spheroids could successfully be initiated on the CAM. Consequently, the CAM assay presents a good base for future studies involving human fibrosarcoma cell spheroids.
纤维肉瘤是软组织肿瘤中一种罕见但极具侵袭性的肿瘤实体。由于其罕见性，关于其发生发展和病理生理学的问题仍不明确。绒毛尿囊膜（CAM）试验是一种在生长膜上对肿瘤进行活体和卵内研究的简便方法。本研究旨在测试纤维肉瘤球体在CAM上是否能够生长，并严格评估其在下游研究中的适用性。打开受精鸡蛋的蛋壳，将预先制备的HT1080细胞球体（每个球体含50000、75000和100000个细胞）应用于CAM。7天后，检查肿瘤的大小、重量和血管生成情况。7天后，163枚鸡蛋中有80枚显示出足够的肿瘤生长。在这80枚确认有肿瘤生长的鸡蛋中，32枚（40%）来自50000细胞球体组，18枚（22.5%）来自75000细胞球体组，30枚（37.5%）来自100000细胞球体组。100000细胞球体组的重量最高，平均为110.7毫克，肿瘤大小也有所扩大。该细胞数量组的血管生成率也最高。纤维肉瘤球体在CAM上能够成功启动肿瘤生长。因此，CAM试验为未来涉及人纤维肉瘤细胞球体的研究提供了良好的基础。
