Pavesi A, Tan A T, Chen M B, Adriani G, Bertoletti A, Kamm R D
Annu Int Conf IEEE Eng Med Biol Soc. 2015;2015:1853-6. doi: 10.1109/EMBC.2015.7318742.
Understanding the mechanism of tumor cell extravasation, cell migration and the role of the immunosystem is crucial in creating targeted and patient-specific cancer therapies. We created an in-vitro microfluidic cell extravasation assay, incorporating a microvascular network and demonstrated its use to study cancer cells extravasation. Separately, we developed an assay for screening T-cell migration and cytotoxicity as a means to evaluate the efficiency of adoptive immunotherapies against cancer. Similar devices using a similar platform can be used to recreate a tumor liver microenvironment, taking in consideration the hypoxic and inflammatory conditions in the liver. These platforms show considerable potential as efficient pre-clinical models for testing the efficiency of cancer drugs and engineered T-cell functionality for personalized medicine.
了解肿瘤细胞外渗、细胞迁移的机制以及免疫系统的作用对于开发靶向性和针对患者的癌症治疗方法至关重要。我们创建了一种体外微流控细胞外渗测定法，其中包含一个微血管网络，并展示了其用于研究癌细胞外渗的用途。另外，我们开发了一种用于筛选T细胞迁移和细胞毒性的测定法，作为评估过继性免疫疗法对抗癌症效率的一种手段。考虑到肝脏中的缺氧和炎症状况，使用类似平台的类似装置可用于重现肿瘤肝脏微环境。这些平台作为测试癌症药物效率和工程化T细胞功能以实现个性化医疗的高效临床前模型具有巨大潜力。
