Horváth M, Schröder D, Varsányi M, Balázsi I
Third Department of Medicine, Semmelweis Medical University, Budapest, Hungary.
Exp Clin Endocrinol. 1989 May;93(2-3):151-6. doi: 10.1055/s-0029-1210849.
In this study 51Cr-labeled chicken erythrocytes coated with human pancreatic extract or rat pancreatic islet homogenate served as target cells for the detection of lymphocyte-mediated cytotoxicity in type I-diabetics. T-lymphocytes from type I-diabetic patients showed a significant cytotoxic capacity against target cells coated with human antigens as well as rat pancreatic islet homogenate. The frequency and intensity of cytotoxicity proved to be similar against the two types of targets. Since rat pancreatic islets are easily available and contain more characteristic beta-cell antigens than human pancreas extract, the homogenate of rat islets of Langerhans is more suitable than human pancreatic extract for studying the lymphocyte-mediated cytotoxicity in type I-diabetic patients.
在本研究中，用人类胰腺提取物或大鼠胰岛匀浆包被的51Cr标记鸡红细胞作为检测I型糖尿病患者淋巴细胞介导细胞毒性的靶细胞。I型糖尿病患者的T淋巴细胞对包被人类抗原以及大鼠胰岛匀浆的靶细胞显示出显著的细胞毒性能力。针对这两种类型靶细胞的细胞毒性频率和强度被证明是相似的。由于大鼠胰岛易于获得且比人类胰腺提取物含有更多特征性β细胞抗原，因此，朗格汉斯大鼠胰岛匀浆比人类胰腺提取物更适合用于研究I型糖尿病患者的淋巴细胞介导细胞毒性。
