Köhler E, Knospe S, Rjasanowski I, Michaelis D
Central Institute of Diabetes Gerhardt Katsch Karlsburg, GDR.
Exp Clin Endocrinol. 1988 Dec;92(2):182-8. doi: 10.1055/s-0029-1210799.
In a two-year follow-up study neonatal rat islets have been shown to be affected in vitro by lymphocytes and complement-inactivated serum obtained from newly diagnosed Type 1 (insulin-dependent) diabetic patients and probands who are at high risk for developing the disease. The effect was measured by 51Cr-release of the islets treated with the proband's serum after a 6 h-incubation with lymphocytes of the same donor. Nineteen newly diagnosed diabetic patients, 23 persons at risk and 11 control probands were studied. There was no appreciable cytotoxic activity in the control probands (with one exception) and in 7 out of the 19 newly diagnosed diabetics. Five of the diabetes-susceptible probands developed diabetes mellitus during the investigation period. Anti-islet cytotoxicity of lymphocytes was found in these individuals at least 8 months before diagnosis of Type 1 diabetes. The cytotoxic effect disappeared at various time intervals after disease manifestation. Islet cytotoxicity was intermittently found with lymphocytes from further 13 probands at risk, sometimes for more than one year. Our data indicate that mononuclear cells from probands who are at high risk for developing Type 1 diabetes can exert cytotoxicity on xenogenic neonatal islets in the presence of their own serum.
在一项为期两年的随访研究中，已表明新生大鼠胰岛在体外会受到从新诊断出的1型（胰岛素依赖型）糖尿病患者以及有患该病高风险的先证者身上获取的淋巴细胞和补体灭活血清的影响。这种影响通过将先证者血清与来自同一供体的淋巴细胞共同孵育6小时后，用51Cr释放法来测定经处理的胰岛的情况。研究了19名新诊断出的糖尿病患者、23名有患病风险的人以及11名对照先证者。在对照先证者（有一个例外）以及19名新诊断出的糖尿病患者中的7人身上未发现明显的细胞毒性活性。在调查期间，5名糖尿病易感先证者患上了糖尿病。在这些个体中，在诊断出1型糖尿病至少8个月前就发现了淋巴细胞的抗胰岛细胞毒性。在疾病显现后的不同时间间隔，细胞毒性作用消失。在另外13名有患病风险的先证者的淋巴细胞中，间歇性地发现了胰岛细胞毒性，有时持续一年多。我们的数据表明，有患1型糖尿病高风险的先证者的单核细胞在其自身血清存在的情况下，可对异种新生胰岛发挥细胞毒性作用。
