Torelli S, Sogos V, Marzilli M A, D'Atri M, Gremo F
Department of Cytomorphology, School of Medicine, Cagliari, Italy.
Exp Biol. 1989;48(4):187-96.
An immunocytochemical study was performed on the chick embryo retina to detect the changes in intermediate filament (IF) composition in correlation with development. Results showed that all three major neurofilament subunits (NF L, M and H) were expressed from very immature neurons at the same time. No delayed expression of NF-H was observed. Vimentin (VT) was also present from very early stages in both neuronal and glial cells, but it was not detectable at hatching. Its distribution changed in retinal layers throughout development. It co-existed with NF in neurons, and conversely, it preceded the appearance of the typical glial fibrillar acidic protein (GFA-P), which was detected in radial fibres of Müller cells from day 16 of incubation. These in vivo data were compared with immunocytochemical staining of retinal cultures, grown either on collagen or on polylysine. NF-L and GFA-P were not detectable in vitro. Moreover, the early expression of NF subunits was shown to be influenced by the type of substratum. The contemporary presence of vimentin and neurofilaments was clearly shown both in neuronal cell bodies and processes. The results also show that in the chick retina the pattern of composition of intermediate filaments changes with maturation. Moreover, in vitro conditions influence the expression of IF components. These data are of interest when performing in vitro analysis of the effect of exogenous substances affecting neuronal maturation and differentiation.
对鸡胚视网膜进行了免疫细胞化学研究，以检测中间丝（IF）组成与发育相关的变化。结果表明，所有三种主要神经丝亚基（NF-L、M和H）同时从非常不成熟的神经元中表达。未观察到NF-H的延迟表达。波形蛋白（VT）在神经元和神经胶质细胞的早期阶段也存在，但在孵化时无法检测到。其分布在整个发育过程中在视网膜各层发生变化。它在神经元中与NF共存，相反，它先于典型的神经胶质纤维酸性蛋白（GFA-P）出现，后者在孵化第16天在Müller细胞的放射状纤维中被检测到。将这些体内数据与在胶原蛋白或聚赖氨酸上生长的视网膜培养物的免疫细胞化学染色进行了比较。在体外无法检测到NF-L和GFA-P。此外，NF亚基的早期表达显示受基质类型的影响。波形蛋白和神经丝在神经元细胞体和突起中均清晰显示同时存在。结果还表明，在鸡视网膜中，中间丝的组成模式随成熟而变化。此外，体外条件影响IF成分的表达。在对外源性物质影响神经元成熟和分化的作用进行体外分析时，这些数据很有意义。
