Bronson R A, Cooper G W, Margalioth E J, Naz R K, Hamilton M S
Laboratory of Human Reproduction, North Shore University Hospital, Cornell University Medical College, New York.
Fertil Steril. 1989 Sep;52(3):457-62. doi: 10.1016/s0015-0282(16)60919-6.
Evolutionarily conserved antigens are present on spermatozoa of several mammalian species. We tested sera from infertile men and women containing antisperm antibodies (ASAs) for their reactivity with FA-1, an antigen known to be present on murine and human spermatozoa. Fifty percent of male sera and 63% of female sera contained anti-FA-1 antibodies, as judged by enzyme linked immunosorbent assay (ELISA). Fourteen percent of male sera and 50% of female sera were also shown to possess ASAs reactive with living mouse spermatozoa, and murine in vitro fertilization was inhibited by human antibodies. These results suggest that the transfer of immunoglobulins from human sera to spermatozoa of other species may provide a model to study how ASAs effect sperm function.
进化上保守的抗原存在于几种哺乳动物的精子上。我们检测了含有抗精子抗体（ASA）的不育男性和女性的血清，以观察其与FA-1的反应性，FA-1是一种已知存在于小鼠和人类精子上的抗原。通过酶联免疫吸附测定（ELISA）判断，50%的男性血清和63%的女性血清含有抗FA-1抗体。还发现14%的男性血清和50%的女性血清具有与活的小鼠精子反应的抗精子抗体，并且人抗体可抑制小鼠的体外受精。这些结果表明，人血清中的免疫球蛋白转移到其他物种的精子上可能为研究抗精子抗体如何影响精子功能提供一个模型。
