Hall J L, Engel D, Naz R K
Department of Obstetrics and Gynecology, George Washington University Medical Center, Washington, DC 20037.
Arch Androl. 1994 Jan-Feb;32(1):25-30. doi: 10.3109/01485019408987763.
A fertilization antigen (FA-1) has been identified on human sperm cells that is involved in human fertilization. To better explain the participation of FA-1 in human infertility, the relationship of anti-FA-1 antibodies with the presence of general antisperm antibodies and with sperm function was examined in the sera of 30 men and women. None of the sera from fertile men and women (n = 10) reacted with FA-1. In contrast, 55% of the immunoinfertile sera (n = 20) that were positive for antisperm antibodies detected by OPTI-BEAD (immune-labeled microsphere) tested positive with FA-1 (p < .05). In these sera, penetration of hamster oocytes was reduced and was inversely related to monospecific anti-FA-1 absorbance values determined by an ELISA and with heterospecific general antisperm antibody binding to sperm in the OPTI-BEAD test (p < .05). Anti-FA-1 activity approached significance with antisperm antibody binding to the sperm head (p = .052). It is concluded that immunoinfertile sera have antibodies reacting with the FA-1 sperm antigen and their presence is significantly related to results of other functional tests, such as the SPA and the OPTI-BEAD test. The association of anti-FA-1 antibodies with antibody binding site (sperm head) may be relevant to the diagnosis and treatment of immunoinfertility.
在人类精子细胞上已鉴定出一种参与人类受精的受精抗原（FA-1）。为了更好地解释FA-1在人类不孕中的作用，研究了30名男性和女性血清中抗FA-1抗体与一般抗精子抗体的存在以及精子功能之间的关系。可育男性和女性（n = 10）的血清均未与FA-1发生反应。相比之下，通过OPTI-BEAD（免疫标记微球）检测出抗精子抗体呈阳性的免疫不孕血清（n = 20）中，55%与FA-1检测呈阳性反应（p <.05）。在这些血清中，仓鼠卵母细胞的穿透率降低，且与通过ELISA测定的单特异性抗FA-1吸光度值以及在OPTI-BEAD试验中异特异性一般抗精子抗体与精子的结合呈负相关（p <.05）。抗精子抗体与精子头部结合时，抗FA-1活性接近显著水平（p = .052）。得出的结论是，免疫不孕血清中存在与FA-1精子抗原反应的抗体，其存在与其他功能试验的结果，如精子穿透试验（SPA）和OPTI-BEAD试验，显著相关。抗FA-1抗体与抗体结合位点（精子头部）的关联可能与免疫性不孕的诊断和治疗有关。
