Lin Lijin, Yang Daiyu, Wang Xun, Liao Ming'an, Wang Zhihui, Lv Xiulan, Tang Fuyi, Liang Dong, Xia Hui, Lai Yunsong, Tang Yi
Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
College of Horticulture, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
Environ Monit Assess. 2016 Feb;188(2):82. doi: 10.1007/s10661-015-5084-3. Epub 2016 Jan 6.
The effects of grafting on the cadmium (Cd) accumulation characteristics of the potential Cd-hyperaccumulator Solanum photeinocarpum were studied under Cd stress in our experiment. Four treatments were used in the experiment: ungrafted (UG), self-rooted grafting by the same S. photeinocarpum seedling (SG), self-rooted grafting by two different development stages of S. photeinocarpum seedlings (DG), and grafting on the rootstock of wild potato (PG). SG and DG decreased the root, scion stem, leaf, whole shoot, and whole plant biomasses compared with UG, but increased the rootstock stem biomass, while only PG increased the root and whole plant biomasses. SG and DG increased the Cd contents in the different organs of S. photeinocarpum compared with UG, while PG decreased the Cd content compared with UG. The Cd extraction by the whole plant of S. photeinocarpum was ranked as DG > SG > UG > PG. Additionally, the antioxidant enzyme activities in SG and DG were enhanced compared with UG, while that of PG was reduced compared with UG. The grafting increased the DNA methylation levels and changed the methylation patterns of S. photeinocarpum compared with UG. Therefore, SG and DG can increase the Cd accumulation in S. photeinocarpum, which can be used for the phytoremediation of Cd-contaminated soil.
在本实验中，研究了嫁接对潜在镉超富集植物少花龙葵镉（Cd）积累特性的影响。实验采用了四种处理方式：未嫁接（UG）、用同一少花龙葵幼苗进行自根嫁接（SG）、用两个不同发育阶段的少花龙葵幼苗进行自根嫁接（DG）以及嫁接到野生马铃薯砧木上（PG）。与UG相比，SG和DG降低了根、接穗茎、叶、地上部和整株生物量，但增加了砧木茎生物量，而只有PG增加了根和整株生物量。与UG相比，SG和DG增加了少花龙葵不同器官中的镉含量，而PG与UG相比降低了镉含量。少花龙葵整株对镉的提取量排序为DG＞SG＞UG＞PG。此外，与UG相比，SG和DG中的抗氧化酶活性增强，而PG与UG相比则降低。与UG相比，嫁接增加了少花龙葵的DNA甲基化水平并改变了甲基化模式。因此，SG和DG可以增加少花龙葵对镉的积累，可用于镉污染土壤的植物修复。
