Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, Sichuan, China.
College of Life Sciences, Sichuan University, Chengdu, China.
Environ Monit Assess. 2019 Sep 5;191(10):609. doi: 10.1007/s10661-019-7787-3.
A study was performed to determine whether self-rooted grafting decreases cadmium (Cd) accumulation in post-grafting soybean (Glycine max (Linn.) Merrill) generations. Pot experiments were performed using ungrafted (UG) seedlings, self-rooted grafting from the same soybean seedling (SG), self-rooted grafting from two soybean seedlings at the same growth stage (TG), and self-rooted grafting from two soybean seedlings at different developmental stages (DG). Growth and Cd accumulation in three post-grafting soybean generations were assessed. In the SG treatment, only the second post-grafting generation had increased shoot biomass and only the first post-grafting generation shoots had decreased Cd contents. The seed Cd content, soluble protein content, and antioxidant enzyme activity were not significantly affected in three post-grafting generations. In the TG and DG treatments, shoot biomass, soluble protein content, and antioxidant enzyme activities were increased, and the shoot and seed Cd contents were decreased in three post-grafting generations. The seed Cd contents in the first, second, and third post-grafting generations were 15.00%, 9.46%, and 12.44%, respectively, lower in the TG than UG treatments. The seed Cd contents in the first, second, and third post-grafting generations were 32.73%, 27.03%, and 32.22%, respectively, lower in the DG than UG treatments. Different grafting methods promoted growth and decreased Cd accumulation to different degrees in three post-grafting generations. Grafting seedlings at different developmental stages had the strongest effects.
一项研究旨在确定自根嫁接是否会降低接后大豆（Glycine max (Linn.) Merrill）世代的镉（Cd）积累。采用未嫁接（UG）幼苗、同一大豆幼苗自根嫁接（SG）、同一生长阶段的两个大豆幼苗自根嫁接（TG）和不同发育阶段的两个大豆幼苗自根嫁接（DG）进行盆栽实验。评估了三个接后大豆世代的生长和 Cd 积累情况。在 SG 处理中，只有第二代接后世代的地上部生物量增加，只有第一代接后世代的地上部 Cd 含量降低。三个接后世代的种子 Cd 含量、可溶性蛋白含量和抗氧化酶活性均无显著影响。在 TG 和 DG 处理中，三个接后世代的地上部生物量、可溶性蛋白含量和抗氧化酶活性均增加，地上部和种子 Cd 含量均降低。与 UG 处理相比，TG 处理的第一代、第二代和第三代接后种子 Cd 含量分别降低了 15.00%、9.46%和 12.44%。与 UG 处理相比，DG 处理的第一代、第二代和第三代接后种子 Cd 含量分别降低了 32.73%、27.03%和 32.22%。不同的嫁接方法在三个接后世代中以不同程度促进了生长并降低了 Cd 积累。嫁接不同发育阶段的幼苗效果最强。
