Yen Tsai-Ying, Trovoada dos Santos Maria de Jesus, Tseng Lien-Feng, Chang Shu-Feng, Cheng Chien-Fu, Carvalho Arlindo Vicente de Assunção, Shu Pei-Yun, Lien Jih-Ching, Tsai Kun-Hsien
Institute of Epidemiology and Preventive Medicine, College of Public Health, National Taiwan University, Taipei, Taiwan.
Department of Health, Sao Tome, Sao Tome and Principe.
Acta Trop. 2016 Mar;155:58-62. doi: 10.1016/j.actatropica.2015.12.012. Epub 2015 Dec 29.
Dengue fever has become a worldwide public health concern, threatening an estimated 40% of the world's population. However, most resources and attention are still focused on malaria, while dengue statuses are poorly recognized in many African countries. In this serological survey, dengue virus (DENV) transmission was demonstrated by using serum samples collected from 78 pregnant women in the Democratic Republic of Sao Tome and Principe (DRSTP) during 2003 to 2004. Immunofluorescence assay was performed and 31 samples (39.74%) were found positive for DENV antibodies. Indirect enzyme-linked immunosorbent assay (ELISA) showed that 53 samples (67.95%) were positive for dengue E IgG, and 38 samples (48.72%) were positive for NS1 IgG. A prevalence of 35.90% was therefore determined for dengue IgG by considering samples that yielded positive results by all three tests. Cross-reactions with other flaviviruses were examined by indirect ELISA against Japanese encephalitis virus, West Nile virus, and yellow fever virus. Only one sample exhibited stronger absorbance against Japanese encephalitis virus and West Nile virus. Moreover, one sample was positive for dengue IgM. These results agreed with the previous researches in neighboring countries and suggested DENV exposure. The study contributes to raising public awareness of dengue and supporting future control strategies.
登革热已成为全球公共卫生问题，威胁着全球约40%的人口。然而，大多数资源和注意力仍集中在疟疾上，而在许多非洲国家，登革热的状况却鲜为人知。在这项血清学调查中，通过使用2003年至2004年期间从圣多美和普林西比民主共和国（DRSTP）的78名孕妇采集的血清样本，证明了登革热病毒（DENV）的传播。进行了免疫荧光测定，发现31份样本（39.74%）的DENV抗体呈阳性。间接酶联免疫吸附测定（ELISA）显示，53份样本（67.95%）的登革热E IgG呈阳性，38份样本（48.72%）的NS1 IgG呈阳性。因此，通过考虑所有三项检测均呈阳性结果的样本，确定登革热IgG的患病率为35.90%。通过针对日本脑炎病毒、西尼罗河病毒和黄热病病毒的间接ELISA检测了与其他黄病毒的交叉反应。只有一份样本对日本脑炎病毒和西尼罗河病毒表现出更强的吸光度。此外，一份样本的登革热IgM呈阳性。这些结果与邻国先前的研究一致，并表明存在DENV暴露。该研究有助于提高公众对登革热的认识，并支持未来的控制策略。
