Jiang Nannan, Yin Jia, Mak Philip, Wen Liping
Department of Allergy; Peking Union Hospital; Peking Union Medical College & Chinese Academy of Medical Science, China.
Department of Biology, Johns Hopkins University, USA.
Iran J Allergy Asthma Immunol. 2015 Oct;14(5):483-92.
Orchard workers in north China are highly exposed to orchard pollens, especially peach and other Rosaceae family pollens during pollination season. The aim of this study was to investigate whether occupational allergy to peach tree pollen as a member of Rosaceae family is IgE-mediated and to evaluate the cross-reactivity among Rosaceae family pollens. Allergen skin test and conjunctival challenge test were performed; enzyme linked immune-sorbent assay (ELISA), inhibiting ELISA, western immunoblotting and inhibiting western immunoblotting were done with Rosaceae family orchard pollens, including peach, apricot, cherry, apple and pear tree pollens. Mass spectrometry was also performed to probe the main allergen component and cross-reactive protein. Sensitizations to peach pollen were found in both skin test and conjunctival challenge in the patients. Serum specific IgE to three pollens (peach, apricot and cherry) were detected through ELISA. When peach pollen used as solid phase, ELISA inhibition revealed other four kinds of pollens capable of inducing partial to strong inhibitions (45% to 87%), with the strongest inhibition belonging to apricot pollen (87%). Western blotting showed predominant IgE binding to a 20 KD protein among these pollens, which appeared to be a cross-reactive allergen component through western blotting inhibition. It was recognized as a protein homologous to glutathione s-transferase 16 from Arabidopsis thaliana. Peach and other Rosaceae family tree pollen may serve as a potential cause of IgE mediated occupational respiratory disease in orchard workers in north China.
中国北方的果园工人在授粉季节高度暴露于果园花粉中，尤其是桃树花粉和其他蔷薇科花粉。本研究的目的是调查作为蔷薇科成员的桃树花粉引起的职业性过敏是否由IgE介导，并评估蔷薇科花粉之间的交叉反应性。进行了过敏原皮肤试验和结膜激发试验；使用包括桃树、杏树、樱桃树、苹果树和梨树花粉在内的蔷薇科果园花粉进行了酶联免疫吸附测定（ELISA）、抑制ELISA、western免疫印迹和抑制western免疫印迹。还进行了质谱分析以探测主要过敏原成分和交叉反应蛋白。在患者的皮肤试验和结膜激发试验中均发现对桃花粉过敏。通过ELISA检测了对三种花粉（桃树、杏树和樱桃树）的血清特异性IgE。当以桃花粉作为固相时，ELISA抑制显示其他四种花粉能够引起部分至强烈抑制（45%至87%），其中抑制作用最强的是杏花花粉（87%）。western印迹显示这些花粉中主要是IgE与一种20KD的蛋白质结合，通过western印迹抑制表明这似乎是一种交叉反应性过敏原成分。它被认为是一种与拟南芥谷胱甘肽s-转移酶16同源的蛋白质。桃树和其他蔷薇科树木花粉可能是中国北方果园工人IgE介导的职业性呼吸道疾病的潜在病因。
