Alachkar Houda, Nakamura Yusuke
a School of Pharmacy, University of Southern California , Los Angeles , CA , USA.
b Section of Hematology/Oncology, Department of Medicine, The University of Chicago , Chicago , IL , USA.
Chimerism. 2015 Jul 3;6(3):47-9. doi: 10.1080/19381956.2015.1128624. Epub 2016 Jan 8.
Haplo-cord transplant has emerged as a feasible and reliable approach for haematopoietic stem cell transplant in patients who are unable to find matched-donor. This approach provides fast myeloid recovery, low incidence of graft vs host disease (GVHD) and favorable graft versus leukemia (GVL) effects. T cell recovery plays an important role in preventing infectious complications; it also mediates the GVHD and the GVL effects. Here, we utilized a novel RNA-based sequencing approach to quantitatively characterize the T cell receptor (TCRs) repertoire in patients underwent haplo-cord transplant in comparison with those underwent matched-donor transplant. Our study shows that higher percentage of cord cells early post transplant were associated with significantly higher TCR diversity. TCR diversity was significantly lower in patients with GVHD and in relapsed patients. A larger cohort study is needed to validate these data and to provide useful information on the specific TCR clones correlated with clinical outcome.
单倍体脐带血移植已成为无法找到匹配供体的患者进行造血干细胞移植的一种可行且可靠的方法。这种方法能使髓系快速恢复，移植物抗宿主病（GVHD）发生率低，且具有良好的移植物抗白血病（GVL）效应。T细胞恢复在预防感染并发症中起重要作用；它还介导GVHD和GVL效应。在此，我们采用一种基于RNA的新型测序方法，对接受单倍体脐带血移植的患者与接受匹配供体移植的患者的T细胞受体（TCR）库进行定量表征。我们的研究表明，移植后早期脐带血细胞比例较高与TCR多样性显著更高相关。GVHD患者和复发患者的TCR多样性显著更低。需要更大规模的队列研究来验证这些数据，并提供与临床结果相关的特定TCR克隆的有用信息。
