Pažoureková Silvia, Lucová Marianna, Nosál Radomír, Drábiková Katarína, Harmatha Juraj, Šmidrkal Ján, Jančinová Viera
Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Dx00FA;bravskx00E1; cesta 9, Bratislava, Slovak Republic.
Pharmacology. 2016;97(3-4):138-45. doi: 10.1159/000443177. Epub 2016 Jan 9.
Equol (7,4'-dihydroxy-isoflavan, or 4',7-isoflavandiol) is a chroman derivative produced by intestinal bacteria in response to soy isoflavone intake in some, but not in all, humans. Equol shows strong anti-oxidant, anti-estrogenic, anti-cancerous and anti-inflammatory properties. The antioxidative capacity of equol has recently received considerable attention, and it has been used for preventing and treating several diseases. We investigated the effect of equol on human neutrophils, extra- and intracellular formation of oxidants, the phosphorylation of protein regulating NADPH oxidase and its effect on apoptosis. Neutrophils, isolated from blood from healthy subjects, were tested upon activation with various stimulants, proper for reactive oxygen species (ROS) production and treated by equol. Equol has the ability to reduce the toxic action of neutrophils. With increasing concentrations, equol decreased the amount of oxidants produced by neutrophils both extra- and intracellularly. The phosphorylation of p40(phox) (a component of NADPH oxidase, responsible for the assembly of functional oxidase in intracellular membranes) was reduced in the presence of equol. The experiments showed that equol did not change the number of viable, apoptotic or dead neutrophils significantly in all concentrations used. These results indicate the promising effect of equol in the operation of ROS in different mechanisms in the model of inflammation.
雌马酚（7,4'-二羟基异黄酮，或4',7-异黄酮二醇）是一种色满衍生物，部分（而非全部）人摄入大豆异黄酮后，肠道细菌会产生这种物质。雌马酚具有强大的抗氧化、抗雌激素、抗癌和抗炎特性。最近，雌马酚的抗氧化能力受到了广泛关注，它已被用于预防和治疗多种疾病。我们研究了雌马酚对人中性粒细胞、氧化剂的细胞外和细胞内形成、调节NADPH氧化酶的蛋白质磷酸化及其对细胞凋亡的影响。从健康受试者血液中分离出的中性粒细胞，在用各种适合产生活性氧（ROS）的刺激剂激活后进行测试，并用雌马酚处理。雌马酚有能力降低中性粒细胞的毒性作用。随着浓度增加，雌马酚减少了中性粒细胞在细胞外和细胞内产生的氧化剂数量。在雌马酚存在的情况下，p40(phox)（NADPH氧化酶的一个组成部分，负责在细胞内膜中组装功能性氧化酶）的磷酸化减少。实验表明，在所有使用的浓度下，雌马酚均未显著改变存活、凋亡或死亡中性粒细胞的数量。这些结果表明，在炎症模型中，雌马酚在ROS的不同机制运作中具有可观的作用。
