Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Dúbravská cesta 9, 841 04 Bratislava, Slovakia.
Oxid Med Cell Longev. 2013;2013:136539. doi: 10.1155/2013/136539. Epub 2013 Oct 31.
Neutrophils are able to release cytotoxic substances and inflammatory mediators, which, along with their delayed apoptosis, have a potential to maintain permanent inflammation. Therefore, treatment of diseases associated with chronic inflammation should be focused on neutrophils; formation of reactive oxygen species and apoptosis of these cells represent two promising targets for pharmacological intervention. Piceatannol, a naturally occurring stilbenoid, has the ability to reduce the toxic action of neutrophils. This substance decreased the amount of oxidants produced by neutrophils both extra- and intracellularly. Radicals formed within neutrophils (fulfilling a regulatory role) were reduced to a lesser extent than extracellular oxidants, potentially dangerous for host tissues. Moreover, piceatannol did not affect the phosphorylation of p40(phox)-a component of NADPH oxidase, responsible for the assembly of functional oxidase in intracellular (granular) membranes. The stilbenoid tested elevated the percentage of early apoptotic neutrophils, inhibited the activity of protein kinase C (PKC)-the main regulatory enzyme in neutrophils, and reduced phosphorylation of PKC isoforms α , β II, and δ on their catalytic region. The results indicated that piceatannol may be useful as a complementary medicine in states associated with persisting neutrophil activation and with oxidative damage of tissues.
中性粒细胞能够释放细胞毒性物质和炎症介质，加上其延迟凋亡，具有维持永久性炎症的潜力。因此，与慢性炎症相关疾病的治疗应集中于中性粒细胞；活性氧的形成和这些细胞的凋亡代表了两个有前途的药物干预靶点。白藜芦醇是一种天然存在的芪类化合物，具有减轻中性粒细胞毒性作用的能力。这种物质减少了中性粒细胞内外产生的氧化剂数量。在中性粒细胞内形成的自由基（发挥调节作用）的减少程度低于细胞外氧化剂，对宿主组织潜在危险。此外，白藜芦醇不影响 NADPH 氧化酶的 p40(phox)-a 成分的磷酸化，该成分负责在细胞内（颗粒）膜中组装功能性氧化酶。测试的芪类化合物提高了早期凋亡中性粒细胞的百分比，抑制了蛋白激酶 C（PKC）的活性-中性粒细胞的主要调节酶，并减少了 PKC 同工型 α 、β II 和 δ 在其催化区域的磷酸化。结果表明，白藜芦醇可能作为一种补充药物，用于与持续的中性粒细胞激活和组织氧化损伤相关的状态。
