Dermpat, Ardooie, Belgium2Department of Dermatology, University Hospital, Ghent, Belgium.
currently in private practice in Maldegem, Belgium.
JAMA Dermatol. 2016 Mar;152(3):312-7. doi: 10.1001/jamadermatol.2015.4766.
Ex vivo dermoscopy (EVD) can be a valuable tool in routine diagnostic dermatopathologic evaluation.
To compare in vivo dermoscopy (IVD) and EVD and to provide guidance for routine dermatopathologic evaluations.
DESIGN, SETTING, AND PARTICIPANTS: This observational study collected 101 consecutive IVD and EVD images of skin tumors from a private dermatology practice from March 1 to September 30, 2013. Four observers (3 dermatologists and 1 dermatopathologist) blinded to the histopathologic diagnoses independently scored and compared the colors, structures, and vessels of EVD images with those of the corresponding IVD images. Data were analyzed from January 1 to March 31, 2014.
Concordance between the EVD and IVD images and gain or loss of colors, structures, and vessels on EVD relative to IVD images.
The final analysis included 404 observations of 101 images. The EVD image was generally similar to the corresponding IVD image but clearly darker, with new areas of blue in 130 of 404 observations (32.2%) and white in 100 of 404 observations (24.8%) and loss of red in 283 of 404 observations (70.0%). Most structures were well preserved. New structureless areas were found in 78 of 404 observations of EVD images (19.3%), and new crystalline structures were detected in 68 of 404 observations of EVD images (16.8%). On EVD images, squames and crusts were lost in 56 of 404 observations (13.9%) and 43 of 404 observations (10.6%), respectively. Blood vessels were lost in 142 of 404 observations of EVD images (35.1%).
The EVD image is an important new tool in dermatopathology and may give direction to targeted tissue processing and examination of skin tumors.
离体共聚焦显微镜（EVD）在常规诊断性皮肤病理评估中可能是一种有价值的工具。
比较体内共聚焦显微镜（IVD）和 EVD，并为常规皮肤病理评估提供指导。
设计、设置和参与者：本观察性研究于 2013 年 3 月 1 日至 9 月 30 日从一家私人皮肤科诊所连续收集了 101 例皮肤肿瘤的 IVD 和 EVD 图像。4 名观察者（3 名皮肤科医生和 1 名皮肤科病理学家）对组织病理学诊断不知情，分别对 EVD 图像的颜色、结构和血管与相应的 IVD 图像进行评分和比较。数据分析于 2014 年 1 月 1 日至 3 月 31 日进行。
EVD 图像与 IVD 图像的一致性，以及 EVD 图像相对于 IVD 图像的颜色、结构和血管的增加或减少。
最终分析包括 101 张图像的 404 个观察结果。EVD 图像通常与相应的 IVD 图像相似，但明显更暗，在 404 个观察结果中有 130 个（32.2%）出现新的蓝色区域，100 个（24.8%）出现新的白色区域，283 个（70.0%）出现红色丢失。大多数结构都得到了很好的保留。在 EVD 图像的 404 个观察结果中有 78 个（19.3%）发现新的无结构区域，在 EVD 图像的 404 个观察结果中有 68 个（16.8%）发现新的结晶结构。在 EVD 图像中，鳞屑和结痂在 404 个观察结果中有 56 个（13.9%）和 43 个（10.6%）丢失，分别为。EVD 图像中血管丢失 142 例（35.1%）。
EVD 图像是皮肤病理学的一个重要新工具，可能为靶向组织处理和皮肤肿瘤检查提供方向。
