Guangdong Provincial Engineering Technology Research Center for Optical Agriculture, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, PR China.
Guangdong Provincial Engineering Technology Research Center for Optical Agriculture, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, PR China.
Biosens Bioelectron. 2016 May 15;79:531-5. doi: 10.1016/j.bios.2015.12.084. Epub 2015 Dec 26.
Herein, we report a simple, one-step reflux method for synthesis of photoluminescent carbon dots (CDs) using citric acid as the carbon source and diethylenetriamine (DETA) as the surface passivation reagent along with a high quantum yield (82.40%), the fluorescence intensity of the CDs was found to be effectively quenched by Hg(II) ions. Upon addition of I(-) to the CDs/Hg(II) complex dispersion, the fluorescence intensity of the CDs was significantly recovered. Furthermore, we developed an "off-on" fluorescence assay for the detection of I(-) using CDs/Hg(II) as a fluorescence probe. This probe enables the selective detection of Hg(II) with a linear range of 0-80 μM and a limit of detection is 0.201 µM and a limit of detection about I(-) is 0.234 µM with a linear range of 0-70 μM. Most importantly, the sensors can be successfully applied to the determination of Hg(II) and I(-) in real lake water and urine of cattles, the "off-on" sensor demonstrates high selectivity, repeatability, stability, which offer this CDs-based "off-on" fluorescent sensor a promising platform for environmental and biological sensing applications.
在这里，我们报告了一种简单的一步回流法，使用柠檬酸作为碳源，二乙烯三胺（DETA）作为表面钝化试剂合成发光明亮的碳点（CDs），具有高量子产率（82.40％）。发现 CDs 的荧光强度可被 Hg（II）离子有效猝灭。向 CDs/Hg（II）配合物分散体中加入 I（-）后，CDs 的荧光强度得到显著恢复。此外，我们开发了一种使用 CDs/Hg（II）作为荧光探针的“关-开”荧光测定法来检测 I（-）。该探针能够选择性地检测 Hg（II），线性范围为 0-80 μM，检测限为 0.201 μM，检测限约为 I（-）的线性范围为 0-70 μM，检测限为 0.234 μM。最重要的是，该传感器可成功应用于实际湖水和牛尿中 Hg（II）和 I（-）的测定，“关-开”传感器表现出高选择性、重复性和稳定性，为基于 CDs 的“关-开”荧光传感器提供了一种有前途的环境和生物传感应用平台。
