Flašker Ajda, Kulkarni Mukta, Mrak-Poljšak Katjuša, Junkar Ita, Čučnik Saša, Žigon Polona, Mazare Anca, Schmuki Patrik, Iglič Aleš, Sodin-Semrl Snezna
Laboratory of Biophysics, Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, SI-1000, Slovenia.
Department of Rheumatology, University Medical Centre Ljubljana, Ljubljana, SI-1000, Slovenia.
J Biomed Mater Res A. 2016 May;104(5):1113-20. doi: 10.1002/jbm.a.35646. Epub 2016 Jan 30.
Nanoscale topography in improving vascular response in vitro was established previously on various titanium surfaces. In the present study different surface nanotopographies that is different diameters of titanium dioxide (TiO2 ) nanotubes (NTs) were fabricated by electrochemical anodization and conditioned with highly reactive gaseous oxygen plasma. The morphology of different diameter NTs was studied by scanning electron microscopy and atomic force microscopy, while changes in chemical composition on the surface before and after plasma treatment were determined by X-ray photoelectron spectroscopy. Performance of human coronary artery endothelial cells (HCAEC) on those conditioned surfaces was studied in regard to cell proliferation, released IL-6 protein and immunofluorescence microscopy (IFM). We show that HCAEC function is dependent on the diameter of the TiO2 NTs, functioning far less optimally when bound to 100 nm TiO2 NTs as compared to Ti foil, 15 nm NTs or 50 nm NTs. There were improved, morphological cell shape changes, observed with IFM, between HCAEC growing on oxygen-rich plasma-treated versus nontreated 100 nm NTs. These endothelialized conditioned Ti nanosurfaces could elucidate optimization conditions necessary for vascular implants in coronary arteries.
先前已证实纳米级形貌可改善体外血管反应，这一现象存在于各种钛表面。在本研究中，通过电化学阳极氧化法制备了具有不同表面纳米形貌（即不同直径的二氧化钛（TiO₂）纳米管（NTs）），并用高活性气态氧等离子体进行处理。通过扫描电子显微镜和原子力显微镜研究了不同直径纳米管的形态，同时利用X射线光电子能谱确定了等离子体处理前后表面化学成分的变化。从细胞增殖、白细胞介素-6（IL-6）蛋白释放以及免疫荧光显微镜（IFM）等方面研究了人冠状动脉内皮细胞（HCAEC）在这些处理过的表面上的性能。我们发现，HCAEC的功能取决于TiO₂纳米管的直径，与钛箔、15nm纳米管或50nm纳米管相比，当与100nm TiO₂纳米管结合时，其功能的发挥远未达到最佳状态。通过IFM观察发现，在富氧等离子体处理过的100nm纳米管上生长的HCAEC与未处理的相比，细胞形态发生了改善。这些内皮化的经处理钛纳米表面可以阐明冠状动脉血管植入物所需的优化条件。
