Ray D, Robert-Lézénès J
INSERM U-248, Faculté de Médecine Lariboisière, Paris, France.
Oncogene Res. 1989;5(1):73-8.
We previously described a c-myc mRNA initiated within intron 1 at a major start site designated P3. This mRNA was observed in tumorigenic murine cells with no detectable rearrangement of the c-myc gene. In the present study, we report that P3 mRNA also coexists with the P1/P2 c-myc transcript in non-tumorigenic murine cell lines and that a c-myc mRNA which appears to be an equivalent of the murine P3 transcript is found in various rat and human cells. These data suggest that P3 represents a promoter of the normal, non-rearranged mammalian c-myc gene. Furthermore, both P1/P2 and P3 mRNA levels are similarly regulated by cycloheximide and by dimethylsulfoxide (DMSO) in murine erythroleukemia Friend cells.
我们之前描述过一种c-myc信使核糖核酸（mRNA），它起始于内含子1内一个被命名为P3的主要起始位点。在c-myc基因无可检测重排的致瘤性鼠细胞中观察到了这种mRNA。在本研究中，我们报告P3 mRNA也与P1/P2 c-myc转录本共存于非致瘤性鼠细胞系中，并且在各种大鼠和人类细胞中发现了一种似乎等同于鼠P3转录本的c-myc mRNA。这些数据表明P3代表正常的、未重排的哺乳动物c-myc基因的一个启动子。此外，在鼠红白血病Friend细胞中，P1/P2和P3 mRNA水平受环己酰亚胺和二甲基亚砜（DMSO）的调控方式相似。
