Daniel Michael G, Lemischka Ihor R, Moore Kateri
Department of Developmental and Regenerative Biology, Icahn School of Medicine, New York, New York.
Black Family Stem Cell Institute, Icahn School of Medicine, New York, New York.
Ann N Y Acad Sci. 2016 Apr;1370(1):24-35. doi: 10.1111/nyas.12989. Epub 2016 Jan 8.
Even though all paradigms of stem cell therapy and regenerative medicine emerged from the study of hematopoietic stem cells (HSCs), the inability to generate these cells de novo or expand them in vitro persists. Initial efforts to obtain these cells began with the use of embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) technologies, but these strategies have yet to yield fully functional cells. Subsequently, more recent approaches involve transcription factor (TF) overexpression to reprogram PSCs and various somatic cells. The induction of pluripotency with just four TFs by Yamanaka informs our ability to convert cell fates and demonstrates the feasibility of utilizing terminally differentiated cells to generate cells with multilineage potential. In this review, we discuss the recent efforts undertaken using TF-based reprogramming strategies to convert several cell types into HSCs.
尽管干细胞治疗和再生医学的所有范例都源自对造血干细胞（HSC）的研究，但仍然无法从头生成这些细胞或在体外进行扩增。获取这些细胞的最初努力始于使用胚胎干细胞（ESC）和诱导多能干细胞（iPSC）技术，但这些策略尚未产生功能完全的细胞。随后，最近的方法涉及通过转录因子（TF）过表达来重编程PSC和各种体细胞。山中伸弥仅用四种TF诱导多能性，这为我们转换细胞命运的能力提供了依据，并证明了利用终末分化细胞生成具有多谱系潜能细胞的可行性。在这篇综述中，我们讨论了最近使用基于TF的重编程策略将几种细胞类型转化为HSC的努力。
