Germec Mustafa, Tarhan Kübra, Yatmaz Ercan, Tetik Nedim, Karhan Mustafa, Demirci Ali, Turhan Irfan
Dept. of Food Engineering, Akdeniz University, Antalya, 07058, Turkey.
Dept. of Food Engineering, Cankiri Karatekin University, Cankiri, 18100, Turkey.
Biotechnol Prog. 2016 Mar;32(2):393-403. doi: 10.1002/btpr.2225. Epub 2016 Mar 29.
Lignocellulosic materials that are the most abundant plant biomass in the world have the potential to become sustainable sources of the produced value added products. Tea processing waste (TPW) is a good lignocellulosic source to produce the value added products from fermentable sugars (FSs). Therefore, the present study is undertaken to produce FSs by using ultrasound-assisted dilute acid (UADA) and dilute acid (DA) hydrolysis of TPW followed by enzymatic hydrolysis. UADA hydrolysis of TPW was optimized by response surface methodology (RSM) at maximum power (900 W) for 2 h. The optimum conditions were determined as 50°C, 1:6 (w/v) solid:liquid ratio, and 1% (w/v) DA concentration, which yielded 20.34 g/L FS concentration. Furthermore, its DA hydrolysis was also optimized by using RSM for comparison and the optimized conditions were found as 120°C, 1:8 solid:liquid ratio, and 1% acid concentration, which produced 25.3 g/L FS yield. Even though the produced sugars with UADA hydrolysis are slightly less, but it can provide significant cost saving due to the lower temperature requirement and less liquid consumption. Besides, enzymatic hydrolysis applied after pretreatments of TPW were very more economic than the conventional enzymatic hydrolysis in the literature due to shorter time requiring. In conclusion, ultrasound-assisted is a promising technology that can be successfully applied for hydrolysis of biomass and can be an alternative to the other hydrolysis procedures and also TPW can be considered as suitable carbon source for the production of value-added products like biofuels, organic acids, and polysaccharides. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:393-403, 2016.
木质纤维素材料是世界上最丰富的植物生物质，有潜力成为生产增值产品的可持续来源。茶叶加工废弃物（TPW）是一种很好的木质纤维素来源，可用于从可发酵糖（FSs）生产增值产品。因此，本研究采用超声辅助稀酸（UADA）和稀酸（DA）水解TPW，随后进行酶水解来生产FSs。通过响应面法（RSM）在最大功率（900 W）下对TPW进行UADA水解2 h。确定最佳条件为50°C、1:6（w/v）固液比和1%（w/v）DA浓度，FS浓度为20.34 g/L。此外，还通过RSM对其DA水解进行了优化以作比较，发现优化条件为120°C、1:8固液比和1%酸浓度，FS产量为25.3 g/L。尽管UADA水解产生的糖略少，但由于所需温度较低且液体消耗较少，可节省大量成本。此外，TPW预处理后进行的酶水解比文献中的传统酶水解经济得多，因为所需时间更短。总之，超声辅助是一种有前途的技术，可成功应用于生物质水解，可替代其他水解程序，并且TPW可被视为生产生物燃料、有机酸和多糖等增值产品的合适碳源。© 2016美国化学工程师学会生物技术进展，32:393 - 403，2016。
