Guneta Vipra, Loh Qiu Li, Choong Cleo
School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.
KK Research Centre, KK Women's and Children Hospital, 100 Bukit Timah Road, Singapore 229899, Singapore.
J Biomed Mater Res A. 2016 May;104(5):1090-101. doi: 10.1002/jbm.a.35644. Epub 2016 Jan 29.
Three dimensional (3D) alginate scaffolds with tunable mechanical and structural properties are explored for investigating the effect of the scaffold properties on stem cell behavior and extracellular matrix (ECM) formation. Varying concentrations of crosslinker (20 - 60%) are used to tune the stiffness, porosity, and the pore sizes of the scaffolds post-fabrication. Enhanced cell proliferation and adipogenesis occur in scaffolds with 3.52 ± 0.59 kPa stiffness, 87.54 ± 18.33% porosity and 68.33 ± 0.88 μm pore size. On the other hand, cells in scaffolds with stiffness greater than 11.61 ± 1.74 kPa, porosity less than 71.98 ± 6.25%, and pore size less than 64.15 ± 4.34 μm preferentially undergo osteogenesis. When cultured in differentiation media, adipose-derived stem cells (ASCs) undergoing terminal adipogenesis in 20% firming buffer (FB) scaffolds and osteogenesis in 40% and 60% FB scaffolds show the highest secretion of collagen as compared to other groups of scaffolds. Overall, this study demonstrates the three-way relationship between 3D scaffolds, ECM composition, and stem cell differentiation.
为了研究支架特性对干细胞行为和细胞外基质（ECM）形成的影响，人们探索了具有可调机械和结构特性的三维（3D）藻酸盐支架。通过使用不同浓度的交联剂（20%-60%）来调整支架制备后的硬度、孔隙率和孔径。在硬度为3.52±0.59 kPa、孔隙率为87.54±18.33%、孔径为68.33±0.88μm的支架中，细胞增殖和脂肪生成增强。另一方面，在硬度大于11.61±1.74 kPa、孔隙率小于71.98±6.25%、孔径小于64.15±4.34μm的支架中的细胞优先进行成骨作用。当在分化培养基中培养时，与其他组的支架相比，在20%固化缓冲液（FB）支架中经历终末脂肪生成且在40%和60% FB支架中经历成骨作用的脂肪干细胞（ASC）显示出最高的胶原蛋白分泌。总体而言，这项研究证明了3D支架、ECM组成和干细胞分化之间的三方关系。
