在科凯恩综合征成纤维细胞中，经日光灯、紫外线和γ射线照射损伤的腺病毒的相对修复情况与着色性干皮病成纤维细胞中的不同。

Relative repair of adenovirus damaged by sunlamp, UV and gamma-irradiation in Cockayne syndrome fibroblasts is different from that in xeroderma pigmentosum fibroblasts.

作者信息

Rainbow A J

出版信息

Photochem Photobiol. 1989 Aug;50(2):201-7. doi: 10.1111/j.1751-1097.1989.tb04148.x.

DOI:10.1111/j.1751-1097.1989.tb04148.x

Abstract

The DNA repair capacities of three unrelated Cockayne syndrome (CS) fibroblast strains were compared to that of three unrelated xeroderma pigmentosum (XP) strains for three different DNA damaging agents using a sensitive host cell reactivation (HCR) technique. Adenovirus type 2 (Ad 2) was treated with either UV light, gamma-rays or sunlamp-irradiation and subsequently assayed for its ability to form viral structural antigens (Vag) in the CS and XP strains using immunofluorescent straining. D37 values for the survival of Ad 2 Vag synthesis in the CS and XP strains, expressed as a percentage of those obtained in normal strains, were used as a measure of DNA repair capacity. Percent HCR values in the XP strains XP25RO, XP2BE and XP5BE respectively were lowest for UV (6, 14 and 6%), intermediate for sunlamp-irradiation (18, 32 and 10%) and highest for gamma-irradiation (65, 61 and 60%), whereas for the CS strains CS1BE, CS3BE and CS278CTO respectively, percent HCR values were lowest for UV (26, 30 and 34%), intermediate for gamma-irradiation (61, 64 and 69%) and near normal for sunlamp-irradiation (82, 73 and 89%). These results suggest that the 'spectrum of lesions' which is defectively repaired in CS is not the same as that which is defectively repaired in XP.

摘要

使用灵敏的宿主细胞再激活（HCR）技术，比较了三株无关的科凯恩综合征（CS）成纤维细胞系和三株无关的着色性干皮病（XP）成纤维细胞系对三种不同DNA损伤剂的DNA修复能力。用紫外线、γ射线或太阳灯照射处理2型腺病毒（Ad 2），随后使用免疫荧光染色法检测其在CS和XP细胞系中形成病毒结构抗原（Vag）的能力。以正常细胞系中Ad 2 Vag合成存活的D37值的百分比来表示CS和XP细胞系中的D37值，以此作为DNA修复能力的衡量指标。XP细胞系XP25RO、XP2BE和XP5BE中，紫外线处理后的HCR百分比值分别最低（6%、14%和6%），太阳灯照射处理后的居中（18%、32%和10%），γ射线照射处理后的最高（65%、61%和60%）；而对于CS细胞系CS1BE、CS3BE和CS278CTO，紫外线处理后的HCR百分比值分别最低（26%、30%和34%），γ射线照射处理后的居中（61%、64%和69%），太阳灯照射处理后的接近正常水平（82%、73%和89%）。这些结果表明，CS中修复缺陷的“损伤谱”与XP中修复缺陷的“损伤谱”不同。