Rainbow A J, Howes M
Mutat Res. 1982 Mar;93(1):235-47. doi: 10.1016/0027-5107(82)90138-5.
The host-cell reactivation of V antigen production for irradiated adenovirus was examined in fibroblasts from 5 unrelated patients with Cockayne's syndrome (CS) and 2 CS heterozygotes. The fibroblast cultures were infected with either irradiated or non-irradiated adenovirus and subsequently examined for the presence of viral structural antigens using immunofluorescent staining. All CS-homozygous strains showed a reduced host-cell reactivation (HCR) of this viral function for both UV-and gamma-irradiated virus. For UV-irradiation of the virus, D37 values expressed as a percentage of that obtained on normal strains, ranged from 14 to 35%. For gamma-irradiation of the virus these values ranged from 61 to 80%. These results indicate some defect in the repair of both UV- and gamma-ray-induced DNA damage for CS. 1 CS-heterozygote strain tested also showed a reduced HCR for UV-irradiated adenovirus intermediate between that of the patient strain and normal, whereas another CS-heterozygote strain showed apparently normal HCR level.
在5名患科凯恩综合征（CS）的不相关患者及2名CS杂合子的成纤维细胞中，检测了经照射的腺病毒V抗原产生的宿主细胞再激活情况。将成纤维细胞培养物用经照射或未经照射的腺病毒感染，随后使用免疫荧光染色检测病毒结构抗原的存在情况。所有CS纯合子菌株对紫外线和γ射线照射的病毒均显示出该病毒功能的宿主细胞再激活（HCR）降低。对于病毒的紫外线照射，以在正常菌株上获得的值的百分比表示的D37值范围为14%至35%。对于病毒的γ射线照射，这些值范围为61%至80%。这些结果表明CS患者在紫外线和γ射线诱导的DNA损伤修复方面存在一些缺陷。所测试的1个CS杂合子菌株对紫外线照射的腺病毒也显示出HCR降低，介于患者菌株和正常菌株之间，而另一个CS杂合子菌株显示出明显正常的HCR水平。
