用于分析脑透析样本中氨基酸神经递质的便携式微芯片电泳荧光检测系统的评估
Evaluation of a Portable Microchip Electrophoresis Fluorescence Detection System for the Analysis of Amino Acid Neurotransmitters in Brain Dialysis Samples.
作者信息
Oborny Nathan J, Costa Elton E Melo, Suntornsuk Leena, Abreu Fabiane C, Lunte Susan M
机构信息
Department of Bioengineering, University of Kansas.
出版信息
Anal Sci. 2016;32(1):35-40. doi: 10.2116/analsci.32.35.
Abstract
A portable fluorescence detection system for use with microchip electrophoresis was developed and compared to a benchtop system. Using this system, six neuroactive amines commonly found in brain dialysate (arginine, citrulline, taurine, histamine, glutamate, and aspartate) were derivatized offline with naphthalene-2,3-dicarboxaldehyde/cyanide, separated electrophoretically, and detected by fluorescence. The limits of detection for the analytes of interest were 50 - 250 nM for the benchtop system and 250 nM - 1.3 μM for the portable system, both of which were adequate for most analyte detection in brain microdialysis samples. The portable system was then demonstrated for the detection of the same six amines in a rat brain microdialysis sample.
摘要
开发了一种用于微芯片电泳的便携式荧光检测系统，并将其与台式系统进行了比较。使用该系统，对脑透析液中常见的六种神经活性胺（精氨酸、瓜氨酸、牛磺酸、组胺、谷氨酸和天冬氨酸）用萘-2,3-二甲醛/氰化物进行离线衍生化，通过电泳分离，并用荧光检测。台式系统对目标分析物的检测限为50 - 250 nM，便携式系统为250 nM - 1.3 μM，这两种检测限对于脑微透析样品中的大多数分析物检测来说都是足够的。然后展示了该便携式系统用于检测大鼠脑微透析样品中的相同六种胺。
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