开发和优化一种基于 PDMS 的集成微透析微芯片电泳装置，具有在线衍生功能，用于连续监测伯胺。
Development and optimization of an integrated PDMS based-microdialysis microchip electrophoresis device with on-chip derivatization for continuous monitoring of primary amines.
机构信息
Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS, USA.
出版信息
Electrophoresis. 2013 Mar;34(6):895-902. doi: 10.1002/elps.201200454. Epub 2013 Feb 26.
Abstract
An all-PDMS on-line microdialysis-microchip electrophoresis with on-chip derivatization and electrophoretic separation for near real-time monitoring of primary amine-containing analytes is described. Each part of the chip was optimized separately, and the effect of each of the components on temporal resolution, lag time, and separation efficiency of the device was determined. Aspartate and glutamate were employed as test analytes. Derivatization was accomplished with naphthalene-2,3,-dicarboxyaldehyde/cyanide (NDA/CN(-)), and the separation was performed using a 15-cm serpentine channel. The analytes were detected using LIF detection.
摘要
本文描述了一种基于全 PDMS 的在线微透析-微芯片电泳联用系统，用于对含伯胺分析物进行实时监测。该系统在芯片上进行衍生化和电泳分离。单独优化了芯片的各个部分，并确定了各组件对装置的时间分辨率、滞后时间和分离效率的影响。使用天冬氨酸和谷氨酸作为测试分析物。衍生化试剂为萘-2,3-二羧酸酐/氰化物（NDA/CN(-)），采用 15cm 蛇形通道进行分离。采用 LIF 检测法检测分析物。
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